Granular formulation of biological entities with improved storage stability
AssigneeThermo Trilogy Corporation
InventorScott C. Silver
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Abstract
A method for preparing granules containing pesticidal or herbicidal biological organisms which can be stored for prolonged periods and conveniently dispensed is provided. Compositions are provided which comprise granules containing entomopathogenic nematodes. Methods for controlling insects, comprising applying the composition to a plant or field is also disclosed.
Claims10
A composition for storage of viable infective juvenile nematodes comprising granules having a soft core consisting essentially of a purified preparation of viable infective juvenile nematodes of a family selected from the group consisting of Steinernematidae, Heterorhabditidae and Phasmarhabditidae supplied in an individual drop of liquid aqueous medium, wherein
- said core is coated with a powdered material
- wherein said aqueous medium within the drop is drawn into the powdered material, causing the powdered material to form a solidified casing surrounding said nematodes
- wherein more than 90% of the infective juvenile nematodes survive when maintained for six weeks at 25.degree. C., or four weeks at 30.degree. C., or use less than 1 ml O.sub.2 per 10.sup.6 nematodes per day when stored at 25.degree. C.
A method for preparing a composition for storage of a purified preparation of viable infective juvenile nematodes of a family selected from the group consisting of Steinernematidae, Heterorhabditidae and Phasmarhabditidae, comprising contacting individual drops of a suspension of said infective juvenile nematodes in a liquid aqueous medium with a powdered material such that said powdered material completely surrounds each of said drops, wherein said aqueous medium within the drops is drawn into the powdered material causing the powdered material to form a solidified casing surrounding said nematodes,
- wherein more than 90% of the infective juvenile nematodes survive when maintained for six weeks at 25.degree. C., or four weeks at 30.degree. C., or use less than 1 ml O.sub.2 per 10.sup.6 nematodes per day when stored at 25.degree. C.
Dependencies are inferred from the claim text.