Composition and method for manufacturing ion selective electrode sensors
AssigneeDade Behring Inc.
InventorsAlan Robert Craig, James David Hamerslag
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Abstract
This invention pertains to fluorophoric compositions and methods of their use for enhancing visualization of various constituents of ion selective electrodes.
Claims9
A multilayer ion-sensitive electrode device for sensing electrolytes in biological fluids, said device having a membrane deposited on a base, the membrane comprising a polymeric matrix having an ionophore and a sufficient amount of a fluorophore dispersed therein,
- said fluorophore being selected from the class of 7-amino-coumarin derivatives having structure shown below:
- ##STR4## where R.sub.1, R.sub.2, R.sub.3, and R.sub.4 are hydrogen, alkyl or alkylene groups, and R.sub.5, and R.sub.6 are hydrogen, alkyl, alkylene, haloalkyl, aryl, halo, carboxyalkyl, oxo-alkyl, or cyano,
- said sufficient amount of the fluorophore adjusted so that the membrane is optically distinguished from the base under ambient lighting when a radiation pattern illuminates the ion-sensitive electrode device.
A multilayer ion-sensitive electrode device for sensing electrolytes in biological fluids, the device having at least two membrane layers deposited on a base, each of said layers comprising a polymeric matrix having an ionophore and a sufficient amount of a fluorophore dispersed therein,
- said fluorophore being selected from the class of 7-amino-coumarin derivatives having structure shown below:
- ##STR5## where R.sub.1, R.sub.2, R.sub.3, and R.sub.4 are hydrogen, alkyl or alkylene groups, and R.sub.5, and R.sub.6 are hydrogen, alkyl, alkylene, haloalkyl, aryl, halo, carboxyalkyl, oxo-alkyl, or cyano,
- said sufficient amount of the fluorophore adjusted so that the membrane layers are optically distinguished from the base under ambient lighting when a radiation pattern illuminates the ion-sensitive electrode device.
A multilayer ion-sensitive electrode device for sensing electrolytes in biological fluids, said device having at least two membrane layers applied successively in a lower and upper relationship on a base, the lower of said layers comprising a polymeric matrix having an ionophore and a sufficient amount of a fluorophore dispersed therein,
- said fluorophore being selected from the class of 7-amino-coumarin derivatives having structure shown below:
- ##STR6## where R.sub.1, R.sub.2, R.sub.3, and R.sub.4 are hydrogen, alkyl or alkylene groups, and R.sub.5, and R.sub.6 are hydrogen, alkyl, alkylene, haloalkyl, aryl, halo, carboxyalkyl, oxo-alkyl, or cyano,
- said sufficient amount of the fluorophore adjusted so that the membrane layers are optically distinguished from the base under ambient lighting when a radiation pattern illuminates the ion-sensitive electrode device,
- the upper of said layers having dispersed therein at least one radiation absorbing agent.
Dependencies are inferred from the claim text.