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AssigneeBoard of Trustees operating Michigan State University
InventorsJohn B. Ohlrogge, Keith R. Roesler, Basil S. Shorrosh
Estimating the term…
An Arabidopsis acetyl-CoA carboxylase (ACCase) gene has been isolated and sequenced. The 10 kb gene encodes a 251 kD cytosolic ACCase isozyme. The nucleic acid sequence of the gene is set forth in SEQ ID No. 1 and has been accorded GenBank Accession No. L27074. The cDNA sequence and deduced amino acid sequence of the cDNA are set forth in SEQ ID Nos. 2 and 3, respectively. By controlling expression of the gene of the present invention, carboxylation of acetyl-CoA to produce malonyl-CoA may be controlled. Thus, by introducing constructs of the gene of the present invention in sense or anti-sense orientation, carboxylation of acetyl-CoA to produce malonyl-CoA may be increased or decreased. Consequently, fatty acid synthesis and elongation in plants and seeds which depends on malonyl-CoA may also be controlled.
An isolated nucleic acid comprising a nucleotide sequence encoding the Arabidosis cytosolic ACCase of SEQ ID No. 3.
A DNA construct comprising a nucleotide sequence encoding the polypeptide of SEQ ID No. 3 operatively-linked to a nucleotide sequence encoding a plastid transit peptide.
A DNA construct comprising a nucleotide sequence encoding a plant cytosolic acetyl-CoA carboxylase operatively-linked to a nucleotide sequence encoding a plastid transit peptide.
A plant transformed with a nucleic acid comprising a nucleotide sequence encoding the polypeptide of SEQ ID No. 3.
Dependencies are inferred from the claim text.