Promoters for enhancing plant productivity
AssigneesThe Regents of the University of California, Institut National de la Recherche Agronomique
InventorsLinda L. Walling, Veronique Pautot, Yong-Qiang Gu, Wun Shaw Chao
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Abstract
Tomato LapA promoters and gene sequences are provided. The promoters are induced in plants upon wounding, or other stress-related conditions. The gene sequences include a full-length LapA gene, particularly the amino terminal region of the gene. Methods of making stress resistant plants, and of making plants susceptible to stress are provided.
Claims18
An isolated recombinant nucleic acid comprising a plant leucine aminopeptidase promoter which remains hybridized to SEQ ID NO:1 or SEQ ID NO:3 at 65.degree. C. in 0.2.times. SSC for 15 minutes.
An isolated recombinant nucleic acid comprising a nucleic acid sequence which encodes a polypeptide comprising residues 1-54 of SEQ ID NO:5.
An isolated full-length recombinant genomic tomato acidic leucine aminopeptidase gene that remains hybridized to SEQ ID NO:1 or SEQ ID NO:3 at 65.degree. C. in 0.2.times. SSC for 15 minutes.
A method of expressing a nucleic acid in response to an environmental stimulus comprising the steps of:
- providing a transgenic plant comprising a recombinant expression cassette having a heterologous nucleic acid operably linked to a recombinant nucleic acid comprising a plant leucine aminopeptidase promoter which hybridizes under stringent conditions to SEQ ID NO:1 or SEQ ID NO:3;
- and,
- subjecting the plant to an environmental stimulus which activates the leucine aminopeptidase promoter, thereby transcribing the heterologous nucleic acid.
Dependencies are inferred from the claim text.