Modified hemoglobin and its use as a component of an artificial blood substitute
AssigneeMontefiore Medical Center
InventorsIraj Lalezari, Parviz Lalezari
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Abstract
The present invention provides a chemically modified form of hemoglobin that is stabilized and can efficiently bind and release oxygen. In addition the chemically modified hemoglobin may be polymerized to increase its molecular weight and increase its stability so that it will have a longer half life in the circulatory system and may be used as a stable oxygen transport mediator which is useful as the basis of a blood substitute.
Claims24
Hemoglobin which is modified by reaction with a compound of the formula:
- wherein R is a bond between the carbon atoms of the phenyl rings;
- --CNH--;
- ##STR11## r is 1, 2 or 3 and B is an active ester moiety capable of reacting with the amino group of a hemoglobin, said active ester moiety being derived from a compound selected from the group consisting of N-hydroxysuccinimide, N-hydroxysulfosuccinimide, N-hydroxyphthalimide and 3,5-dibromosalicylic acid.
Hemoglobin which is modified by reaction with a compound of the formula:
- ##STR12## wherein R is a bond between the carbon atoms of the phenyl rings;
- --CNH--;
- ##STR13## n is 1 or 2 ;
- r is 1, 2 or 3 and B is an active ester moiety capable of reacting with the amino group of a hemoglobin said active ester moiety being derived from a compound selected from the group consisting of N-hydroxysuccinimide, N-hydroxysulfosuccinimide, N-hydroxyphthalimide and 3,5-dibromosalicylic acid.
Hemoglobin which is modified by reaction with a compound of the formula:
- ##STR14## wherein R is a bond between the carbon atoms of the phenyl rings;
- --CNH--;
- ##STR15## n is 1 or 2;
- r is 1, 2 or 3 and B is an active ester moiety capable of reacting with the amino group of a hemoglobin, said active ester moiety being derived from a compound selected from the group consisting of N-hydroxysuccinimide, N-hydroxysulfosuccinimide, N-hydroxyphthalimide and 3,5-dibromosalicylic acid.
Hemoglobin which is modified by reaction with a compound of the formula:
- ##STR16## wherein A is a bond between the carbon atoms of the phenyl rings, CH.sub.2 ;
- ##STR17## --S--;
- --SO--;
- --SO.sub.2 --;
- --CH.sub.2 CH.sub.2 --;
- or --CH.dbd.CH--. Q is --NH--, a bond between the carbon of the phenyl ring and the carbonyl group;
- or --CH.sub.2 --;
- and B' is H or is the same as B provided that at least one B' is B, r is 1, 2 or 3 and B is an active ester moiety capable of reacting with the amino group of a hemoglobin, said active ester moiety being derived from a compound selected from the group consisting of N-hydroxysuccinimide, N-hydroxysulfosuccinimide, N-hydroxyphthalimide and 3,5-dibromosalicylic acid.
Hemoglobin which is modified by reaction with a compound of the formula:
- ##STR18## wherein B is an active ester moiety capable of reacting with the amino group of a hemoglobin, said active ester moiety being derived from a compound selected from the group consisting of N-hydroxysuccinimide, N-hydroxysulfosuccinimide, N-hydroxyphthalimide and 3,5 dibromosalicylic acid and r is 1, 2 or 3;
- and m is 3.
Hemoglobin which is modified by reaction with a compound of the formula:
- ##STR19## wherein B is an active ester moiety capable of reacting with the amino group of a hemoglobin, said active ester moiety being derived from a compound selected from the group consisting of N-hydroxysuccinimide, N-hydroxysulfosuccinimide, N-hydroxyphthalimide and 3,5 dibromosalicylic acid and r is 1, 2 or 3.
Dependencies are inferred from the claim text.