Process for purifying recombinant human serum albumin
AssigneeYoshitomo Pharmaceutical Industries, Ltd.
InventorsMunehiro Noda, Akinori Sumi, Takao Ohmura, Kazumasa Yokoyama
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Abstract
The invention provides a process for purifying recombinant human serum albumin (rHSA) by heating a culture medium containing rHSA and the rHSA-producing host cello, feeding said heated solution upwardly into a fluidized bed in which adsorbent particles are suspended to effect contacting with the adsorbent particles and then recovering the adsorbed fraction containing the rHSA, and a composition comprising rHSA which shows a A35D/A280 ratio of below 0.015, when formulated into a 25% solution of said albumin.
Claims8
A process for purifying recombinant human serum albumin which comprises:
- heating a culture medium containing said albumin and recombinant human serum albumin-producing host cells to inactivate a protease in said culture medium, contacting said heated culture medium with adsorbent particles suspended in a fluidized bed under conditions for selectively adsorbing said albumin and recovering the adsorbed fraction;
- and
- subjecting said recovered adsorbed fraction to at least one treatment selected from the group consisting of heat treatment in the presence of a reducing agent, hydrophobic chromatography, anion exchanger treatment, chelate resin treatment, boric acid/borate treatment and ultrafiltration membrane treatment;
- said purified recombinant human serum albumin having an A350/A280 ratio of below 0.015, as measured in a 250 mg/ml solution of said human serum albumin.
Dependencies are inferred from the claim text.