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US 5,962,642 AGranted
Method for sequencing of protein or peptide
AssigneeSeiko Instruments Inc.
InventorsAkira Tsugita, Keiji Takamoto, Tatsuaki Ataka, Toshihiko Sakuhara, Toyoaki Uchida
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Abstract
A protein or peptide which has an amino-terminus serine or threonine which has an acetylated alpha-amino group is allowed to react with an acid, and then allowed to react with an isothiocyanate under acidic conditions to thereby obtain a thiocarbamyl compound. Then the compound is to be analyzed using Edman degradation. Analysis can be performed with fewer operation steps and without using enzymes.
Claims26
A method for the sequencing of a protein or a peptide, comprising the steps of:
- treating one of a first protein and a first peptide, the selected one of which has an amino-terminal amino acid residue having an acetylated .alpha.-amino group, with an acid to form one of a second protein or a second peptide each of which has an amino-terminal amino acid residue having an acetylated hydroxyl group,
- treating one of the second protein or the second peptide with an isothiocyanate compound in the presence of an acid surrounding the selected one of the second protein or the second peptide to form a thiocarbamyl protein or peptide;
- and
- treating the thiocarbamyl protein or peptide by Edman degradation.
1 more element
A method for the sequencing of a protein or a peptide comprising the steps of:
- treating one of a first protein and a first peptide, the selected one of which has an amino-terminal amino acid residue having an acetylated .alpha.-amino group, with an acid to form one of a second protein and a second peptide, each of which has an amino-terminal amino acid residue having an acetylated hydroxyl group;
- treating one of the second protein and the second peptide with an isothiocyanate compound to form a thiocarbamyl protein or peptide;
- and
- treating the thiocarbamyl protein or peptide by Edman degradation.
1 more element
Dependencies are inferred from the claim text.