Storage-stable fibrinogen preparations
AssigneeImmuno Aktiengesellschaft
InventorThomas Seelich
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Abstract
The invention relates to storage-stable fibrinogen preparations for preparing concentrated fibrinogen solution for use as a tissue adhesive or for preparing fibrinogen solutions for other uses, for example, for infusion purposes. The fibrinogen preparations are characterized in that (i) the lyophilized preparation comprises a substance improving the solubility of fibrinogen such that the reconstitution time is up to 15 minutes, preferably less than 7 minutes, when dissolving with water at room temperature to a solution with a fibrinogen concentration of at least 70 mg/ml and (ii) the ready-to-use tissue adhesive solution obtained from the preparation forms fibrin clots having physiological fibrin structure after mixing with a thrombin-CaCl.sub.2 solution.
Claims25
A lyophilized storage-stable fibrinogen preparation, wherein
- (i) the lyophilized storage-stable preparation comprises a substance for improving the solubility of fibrinogen such that reconstitution time is no longer than 15 minutes when dissolving with water at room temperature to form a solution with a fibrinogen concentration of at least 70 mg/ml, wherein the substances improving the solubility of fibrinogen is at least one selected from the group consisting of:
- vitamins,
- nucleic bases, nucleosides and nucleotides,
- piperidine, pyrimidine, morpholine, pyrrole, imidazole, pyrazole, furan, thiazole and purine, benzoic acid, p-aminobenzoic acid (vitamin H'), p-aminosalicyclic acid, hydroxybenzoic acid, hydroxysalicyclic acid, hydroxypyridines, pyridine dicarboxylic acids, and pyridinesulfonic acid;
- and wherein
- (ii) a tissue adhesive solution obtained from the lyophilized storage-stable preparation forms fibrin clots having physiological fibrin structure after mixing with a solution comprising thrombin and CaCl.sub.2.
A deep-frozen storage-stable fibrinogen preparation in, wherein
- (i) the deep-frozen preparation comprises a substance for improving the solubility of fibrinogen such that the preparation is liquifiable to a solution having a fibrinogen concentration of at least 70 mg/ml at a temperature of 0 to 25.degree. C., wherein the substance improving the solubility of fibrinogen is at least one selected from the group consisting of:
- vitamins,
- nucleic bases, nucleosides and nucleotides,
- piperidine, pyrimidine, morpholine, pyrrole, imidazole, pyrazole, furan, thiazole and purine, benzoic acid, p-aminobenzoic acid, (vitamin H'), p-aminosalicyclic acid, hydroxybenzoic acid, hydroxysalicyclic acid, hydroxypyridines, pyridine dicarboxylic acids, and pyridinesulfonic acid;
- and wherein
- (ii) a tissue adhesive solution obtained from the lyophilized storage-stable preparation forms fibrin clots having physiological fibrin structure after mixing with a solution comprising thrombin and CaCl.sub.2.
A lyophilized storage-stable fibrinogen preparation, wherein
- (i) the preparation comprises a substance improving the solubility of fibrinogen in order that the reconstitution time is no longer than 15 minutes when dissolving with water at room temperature to form a solution having a fibrinogen concentration of at least 70 mg/ml, wherein said substance improving the solubility of fibrinogen is selected from the group consisting of phenylalanine, procaine, niacin, niacinamide, picolinic acid, vitamin B.sub.6 (pyridoxine), piperidine carboxylic acid ester, pyrimidine, barbituric acid, uracil, uridine, uridine phosphates, thymine, cytosine, cytidine, hydroxypyrimidines, thiamine (vitamin B.sub.1), morpholine, pyrrolidone, imidazol, histidine, hydantoin, pyrazole dicarboxylic acid, phenazone, adenosine, adenosine phosphates, inosine, guanosine phosphates, .alpha.-furoic acid (furan-2-carboxylic acid), ascorbic acid (vitamin C) and xantosine, and
- (ii) the tissue adhesive solution obtained from the preparation forms fibrin clots having physiological fibrin structure after mixing with a thrombin-CaCl.sub.2 solution.
A lyophilized fibrinogen preparation containing a substance for improving the solubility of fibrinogen, wherein the substance is at least one selected from the group consisting of benzoic acid, p-aminobenzoic acid, (vitamin H'), p-aminosalicyclic acid, hydroxybenzoic acid, hydroxysalicyclic acid, hydroxypyridines, pyridine dicarboxylic acids, pyridinesulfonic acid, piperidine, pyrimidine, morpholine, pyrrole, imidazole, pyrazole, furan, thiazole and purine, wherein the substance has no cytotoxic effect at the concentration present in the preparation.
A lyophilized fibrinogen preparation comprising at least one vitamin for improving the solubility of fibrinogen.
A lyophilized fibrinogen preparation comprising at least one substance selected from the soup consisting of nucleic bases, nucleosides and nucleotides, wherein the substance improves the solubility of fibrinogen.
A lyophilized fibrinogen preparation comprising a substance for improving the solubility of fibrinogen, wherein the substance for improving the solubility of fibrinogen is selected from the group consisting of benzoic acid, phenylalanine, procaine, niacin, niacinamide, picolinic acid, vitamin B.sub.6 (pyridoxine), piperidine carboxylic acid ester, pyrimidine, barbituric acid, uracil, uridine, uridine phosphates, thymine, cytosine, cytidine, hydroxypyrimidines, thiamine (vitamin B.sub.1) morpholine, pyrrolidone, imidazol, histidine, hydantoin, pyrazole dicarboxylic acid, phenazone, adenosine, adenosine phosphates, inosine, guanosine phosphates, .alpha.-furoic acid (furan-2-carboxylic acid), ascorbic acid (vitamin C) and xantosine.
A lyophilized fibrinogen preparation comprising fibrinogen and a compound for improving the solubility of fibrinogen, wherein the compound is at least one selected from the group consisting of benzoic acid, p-aminobenzoic acid (vitamin H'), p-aminosalicyclic acid, hydroxybenzoic acid, hydroxysalicyclic acid, hydroxypyridines, pyridine dicarboxylic acids, pyridinesulfonic acid, piperidine, pyrimidine, morpholine, pyrrole, imidazole, pyrazole, furan, thiazole, vitamins purine, nucleic bases, nucleosides and nucleotides.
A preparation comprising a compound for improving the solubility of fibrinogen, wherein the compound is at least one selected from the group consisting of benzoic acid, phenylalanine, procaine, niacin, niacinamide, picolinic acid, vitamin B.sub.6 (pyridoxine), piperidine carboxylic acid ester, pyrimidine, barbituric acid, uracil, uridine, uridine phosphates, thymine, cytosine, cytidine, hydroxypyrimidines, thiamine (vitamin B.sub.1), morpholine, pyrrolidone, imidazol, histidine, hydantoin, pyrazole dicarboxylic acid, phenazone, adenosine, adenosine phosphates, inosine, guanosine phosphates, .alpha.-furoic acid (furan-2-carboxylic acid), ascorbic acid (vitamin C) and xantosine.
A frozen fibrinogen preparation comprising fibrinogen and a compound for improving the solubility of fibrinogen fibrinogen, wherein the compound is at least one selected from the group consisting of benzene, pyridine, piperidine, pyrimidine, morpholine, pyrrole, imidazole, pyrazole, furan, thiazole, vitamins, purine nucleic bases, nucleosides and nucleotides.
A frozen fibrinogen preparation comprising a compound for improving the solubility of fibrinogen, wherein the compound is at least one selected from the group consisting of benzoic acid, phenylalanine, procaine, niacin, niacinamide, picolinic acid, vitamin B.sub.6 (pyridoxine), piperidine carboxylic acid ester, pyrimidine, barbituric acid, uracil, uridine, uridine phosphates, thymine, cytosine, cytidine, hydroxypyrimidines, thiamine (vitamin B.sub.1), morpholine, pyrrolidone, imidazol, histidine, hydantoin, pyrazole dicarboxylic acid, phenazone, adenosine, adenosine phosphates, inosine, guanosine phosphates, a-furoic acid (firan-2-carboxylic acid), ascorbic acid (vitamin C) and xantosine.
Dependencies are inferred from the claim text.