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OwnerAtsushi Shimada, Miki Odate +4inventors
InventorsAtsushi Shimada, Miki Odate, Nobuto Koyama, Kimikazu Hashino, Kiyozo Asada, Ikunoshin Kato
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An isolated SDS-resistant hyperthermostable .beta.-galactosidase gene derived from Pyrococcus furiosus. Examples thereof include genes respectively shown in SEQ ID NO: 1 and SEQ ID NO: 2 and genes each hybridizable with the above gene shown in SEQ ID NO: 1. A method of cloning the hyperthermostable .beta.-galactosidase gene in which each of the above genes or pacts thereof is used as a probe or primer. A process for producing a hyperthermostable .beta.-galactosidase by culturing a transformant into which a plasmid containing each of the above gene has been introduced.
An isolated DNA encoding a polypeptide possessing .beta.-galactosidase activity of about 80% or above after being subjected to heat treatment at 90.degree. C. for 120 minutes, and having a DNA sequence which hybridizes with a DNA sequence complementary to the DNA sequence shown in SEQ ID NO. 2 under stringent conditions.
An isolated DNA encoding a polypeptide possessing SDS-resistant .beta.-galactosidase activity of about 80% or above after being subjected to heat treatment at 90.degree. C. for 120 minutes in the presence of 1% SDS, and having a DNA sequence which hybridizes with a DNA sequence complementary to the DNA sequence shown in SEQ ID NO. 4 under stringent conditions.
A method of cloning a DNA having a sequence encoding a polypeptide possessing SDS-resistant .beta.-galactosidase activity of about 80% or above after being subjected to heat treatment at 90.degree. C. for 120 minutes in the presence of 1% SDS, which comprises:
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