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US 5,962,324 AGranted
Three dimensional optic tissue culture and process
AssigneeThe United States of America as represented by the Administrator of the National Aeronautics and Space Adminstration
InventorsKim C. O'Connor, Glenn F. Spaulding, Thomas J. Goodwin, Laurie A. Aten, Karen M. Francis, Delmar R. Caldwell, Tacey L. Prewett, Wendy S. Fitzgerald
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Abstract
A process for artificially producing three-dimensional optic tissue has been developed. The optic cells are cultured in a bioreactor at low shear conditions. The tissue forms as normal, functional tissue grows with tissue organization and extracellular matrix formation.
Claims22
A process for producing aggregates of optic cells comprising the steps of:
- (a) selecting the optic cells for culture;
- (b) introducing the cells and a cell attachment means and a culture media into a cylindrical culture vessel that rotates about its central horizontal axis;
- and
- (c) culturing the cells in the vessel during horizontal rotation modulated to create low shear conditions whereby extra-cellular, optic tissue matrixes are formed and three dimensional cell growth is achieved.
1 more element
An artificially produced optic tissue made by the steps of:
- (a) selecting optic cells for culture;
- (b) introducing the cells and a cell attachment means and a culture media into a cylindrical culture vessel that rotates about its central horizontal axis;
- and
- (c) culturing the cells in the vessel during horizontal rotation modulated to create low shear conditions whereby extra cellular matrixes are formed and three dimensional tissue growth is achieved.
1 more element
Dependencies are inferred from the claim text.