Expansion of bone marrow stromal cells
AssigneeALG, Inc.
InventorsJoel S. Greenberger, David R. Hurwitz
Life of this patent
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Abstract
The invention features a method for the selection and expansion of bone marrow stromal cells. The method includes the steps of obtaining bone marrow stromal cells; introducing the stromal cells into a vessel pre-coated on an inner surface with a gelatin, and containing a culture medium including an acidic fibroblast growth factor ("aFGF") polypeptide; and expanding the stromal cells in the culture medium under conditions and for a time sufficient to obtain an increased number of bone marrow stromal cells. The culture medium additionally can include heparin, and the vessel additionally can be precoated with fetal bovine serum.
Claims14
A method for the expansion of bone marrow stromal cells, the method comprising:
- (a) introducing bone marrow stromal cells into a vessel containing a culture medium comprising an acidic fibroblast growth factor ("aFGF") polypeptide;
- and
- (b) expanding the stromal cells in the culture medium under conditions and for a time sufficient to obtain an increased number of bone marrow stromal cells.
Dependencies are inferred from the claim text.