DNA encoding p107 tumor suppressor and related polypeptides
AssigneeDana-Farber Cancer Institute, Inc.
InventorsDavid M. Livingston, Mark E. Ewen
Life of this patent
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Abstract
A cDNA encodes p107; a cell contains recombinant p107-encoding DNA; and substantially all of the cells of a nonhuman mammal contain recombinant p107-encoding DNA. Also, a method for diagnosing a condition of tumorigenicity in a subject, includes the steps of obtaining a tissue sample from the subject and detecting the presence of non wild-type p107-encoding gene in the sample, or detecting the absence of wild-type p107-encoding gene in the sample; or extracting DNA from the sample and detecting the presence of non wild-type p107-encoding gene or the absence of wild-type p107-encoding gene in the DNA. Also, a nucleic acid probe is complementary to a portion of a human mutant p107 gene.
Claims24
A vector comprising a nucleic acid whose nucleotide sequence includes a region that encodes a polypeptide whose sequence comprises SEQ ID NO:2.
A probe comprising a nucleic acid whose nucleotide sequence includes a region that encodes a polypeptide whose amino acid sequence comprises SEQ ID NO:2.
An isolated cDNA whose nucleotide sequence includes a region that encodes a polypeptide whose amino acid sequence comprises SEQ ID NO:2.
A vector comprising a nucleic acid that encodes a polypeptide comprising residues 225-816 of SEQ ID NO:2.
A probe comprising a nucleic acid that encodes a polypeptide comprising residues 225-816 of SEQ ID NO:2.
An isolated cDNA that encodes a polypeptide comprising residues 225-816 of SEQ ID NO:2.
A vector comprising a nucleic acid whose nucleotide sequence includes a first sequence that encodes a first polypeptide comprising SEQ ID NO:13 and a second sequence that encodes a second polypeptide comprising SEQ ID NO:14, the first and second sequences being separated from one another by a 600 nucleotide spacer, which spacer encodes a polypeptide that binds cyclin A.
A probe comprising a nucleic acid whose nucleotide sequence includes a first sequence that encodes a first polypeptide comprising SEQ ID NO:13 and a second sequence that encodes a second polypeptide comprising SEQ ID NO:14, the first and second sequences being separated from one another by a 600 nucleotide spacer, which spacer encodes a polypeptide that binds cyclin A.
An isolated cDNA whose nucleotide sequence includes a first sequence that encodes a first polypeptide comprising SEQ ID NO:13 and a second sequence that encodes a second polypeptide comprising SEQ ID NO:14, the first and second sequences being separated from one another by a 600 nucleotide spacer, which spacer encodes a polypeptide that binds cyclin A.
A vector comprising a nucleic acid whose nucleotide sequence includes a first sequence that encodes a first polypeptide comprising SEQ ID NO:23 and a second sequence that encodes a second polypeptide comprising SEQ ID NO:25, the first and second sequences being separated from one another by a 588 nucleotide spacer.
A probe comprising a nucleic acid whose nucleotide sequence includes a first sequence that encodes a first polypeptide comprising SEQ ID NO:23 and a second sequence that encodes a second polypeptide comprising SEQ ID NO:25, the first and second sequences being separated from one another by a 589 nucleotide spacer.
An isolated cDNA whose nucleotide sequence includes a first sequence that encodes a first polypeptide comprising SEQ ID NO:23 and a second sequence that encodes a second polypeptide comprising SEQ ID NO:25, the first and second sequences being separated from one another by a 588 nucleotide spacer.
A cDNA that is identical in nucleotide sequence to a fragment of SEQ ID NO:1 and hybridizes specifically to one strand of SEQ ID NO:1 in that, when the fragment is contacted with the strand in the presence of 6.times. SSC, 5.times. Denhardt's, 20 mM NaPO.sub.4, 20% formamide, 0.1% SDS, and 100 .mu.g/ml denatured, sonicated salmon sperm DNA, at 42.degree. C. for 12-18 hours, the fragment binds to the strand.
A cell transformed with a nucleic acid selected from the group consisting of;
- (i) nucleic acid whose nucleotide sequence includes a region that encodes a polypeptide whose amino acid sequence comprises SEQ ID NO:2;
- (ii) a nucleic acid that encodes a polypeptide comprising residues 225-816 of SEQ ID NO:2;
- (iii) a nucleic acid whose nucleotide sequence includes a first sequence that encodes a first polypeptide comprising SEQ ID NO:13 and a second sequence that encodes a second polypeptide comprising SEQ ID NO:14, the first and second sequences being separated from one another by a 600 nucleotide spacer, which spacer encodes a polypeptide that binds cyclin A;
- (iv) a nucleic acid whose nucleotide sequence includes a first sequence that encodes a first polypeptide comprising SEQ ID NO:23 and a second sequence that encodes a second polypeptide comprising SEQ ID NO:25, the first and second sequences being separated from one another by a 588 nucleotide spacer.
A vector comprising a nucleic acid that encodes a polypeptide comprising a fragment of SEQ ID NO:2, the fragment being selected from the group consisting of SEQ ID NO:3, SEQ ID NO:4, and SEQ ID NO:5.
A probe comprising a nucleic acid that encodes a polypeptide comprising a fragment of SEQ ID NO:2, the fragment being selected from the group consisting of SEQ ID NO:3, SEQ ID NO:4, and SEQ ID NO:5.
A cDNA that encodes a polypeptide comprising a fragment of SEQ ID NO:2, the fragment being selected from the group consisting of SEQ ID NO:3, SEQ ID NO:4, and SEQ ID NO:5.
A vector comprising a nucleic acid whose nucleotide sequence is selected from the group consisting of SEQ ID NO:6, SEQ ID NO:7, and SEQ ID NO:8.
A probe comprising a nucleic acid whose nucleotide sequence is selected from the group consisting of SEQ ID NO:6, SEQ ID NO:7, and SEQ ID NO:8.
A cDNA whose nucleotide sequence is selected from the group consisting of SEQ ID NO:6, SEQ ID NO:7, and SEQ ID NO:8.
Dependencies are inferred from the claim text.