Topoisomerase III
AssigneeSmithKline Beecham Corporation
InventorsMichael N. Gwynn, Howard Kallendar
Life of this patent
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Abstract
Topoisomerase III polypeptides and DNA and RNA encoding such Topoisomerase III polypeptides and a procedure for producing such polypeptides by recombinant techniques is disclosed. Also disclosed are methods for utilizing such Topoisomerase III for the treatment of infection, particularly bacterial infections. Antagonists against such Topoisomerase III and their use as a therapeutic to treat infections, particularly bacterial infections are also disclosed. Also disclosed are diagnostic assays for detecting diseases related to the presence of Topoisomerase III nucleic acid sequences and the polypeptides in a host. Also disclosed are diagnostic assays for detecting polynucleotides encoding Streptococcal Topoisomerase III and for detecting the polypeptide in a host.
Claims29
An isolated polynucleotide segment comprising:
- a first polynucleotide sequence, wherein the first polynucleotide sequence (a) is a reference sequence that encodes the amino acid sequence set forth in SEQ ID NO:2, or (b) has at least 95% identity with the reference sequence, wherein identity is determined using an algorithm consisting of BLASTN, where the algorithm is adapted to give the largest match between sequences tested, over the entire length of the reference sequence.
An isolated polynucleotide segment comprising a first polynucleotide sequence, wherein the first polynucleotide sequence is (a) a reference sequence which encodes the same mature polypeptide, expressed by the Topoisomerase III gene contained in Streptococcus pneumoniae 0100993 contained in NCIMB Deposit No. 40794, or (b) has at least 95% identity with the reference sequence, wherein identity is determined using an algorithm consisting of BLASTN, where the algorithm is adapted to give the largest match between sequences tested, over the entire length of the reference sequence.
A recombinant polynucleotide segment comprising nucleotides 1 to 441 of the polynucleotide sequence set forth in SEQ ID NO:1, or the full complement of the entire length of nucleotides 1 to 441 set forth in SEQ ID NO:1.
A recombinant polynucleotide segment which encodes a polypeptide comprising a region having the amino acid sequence of SEQ ID NO:2.
An isolated polynucleotide comprising:
- (a) a first polynucleotide sequence, wherein the first polynucleotide sequence is selected from the group consisting of:
- (i) a first reference sequence which encodes the amino acid sequence set forth in SEQ ID NO:2;
- (ii) a second reference sequence which is nucleotides 1 to 441 set forth in SEQ ID NO:1;
- (iii) a third reference sequence which comprises at least 50 sequential bases of the first reference sequence or the second reference sequence;
- and
- (iv) a fourth reference sequence which comprises at least 30 sequential bases of the first reference sequence or the second reference sequence;
- or
- (b) the full complement of the first polynucleotide sequence.
Dependencies are inferred from the claim text.