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AssigneeKabushiki Kaisha Hayashibara Seibutsu Kagaku Kenkyujo
InventorsKeiji Tsusaki, Michio Kubota, Hiroto Chaen
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Disclosed are a polypeptide having a .beta.-fructofuranosidase activity obtainable by the expression of a microbial gene, a DNA encoding the polypeptide, a transformant obtained by introducing the DNA into an appropriate host, a process for producing the polypeptide comprising culturing the transformant to produce the polypeptide and collecting the produced polypeptide from the culture, and a method for fructofuranosyl transfer comprising a step of reacting a fructofuranosyl donor with a fructofuranosyl acceptor in the presence of the polypeptide.
An isolated polypeptide having .beta.-fructofuranosidase activity, which is obtainable by the expression of a Bacillus gene, contains the amino acid sequences of SEQ ID NOs:
A polypeptide comprising the sequence of SEQ ID NO:3.
An isolated polypeptide comprising the amino acid sequences of SEQ ID NO:
Dependencies are inferred from the claim text.
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No related US applications are recorded in the indexed filing history.
Preparation of compounds containing saccharide radicals: produced by the action of a carbohydrase , e.g. by alpha-amylase , e.g. by cellulase,…
Acting on alpha -1,4-glucosidic bonds: beta-fructofuranosidase , i.e. invertase
Preparation of compounds containing saccharide radicals: produced by the action of a glycosyl transferase, e.g. alpha-, beta- or gamma-cyclodextrins
Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds: beta-fructofuranosidase , i.e. invertase
Term ended 6 Jun 2017 (estimated)
USPTO file history isn’t available for this patent, so only indexed dates are shown.
1. Field of the Invention
The present invention relates to a polypeptide having an enzymatic activity, more particularly, to a polypeptide having a .beta.-fructofuranosidase activity obtainable by the expression of a microbial gene.
2. Description of the Prior Art
FIG. 1 is a restriction map of the recombinant DNA PGGF4 according to the present invention.
FIG. 2 is a figure of the optimum pH of the polypeptide in Example of the present invention.
FIG. 3 is a figure of the optimum temperature of the polypeptide in Example of the present invention.
Now explaining the present invention, the wording "polypeptide(s)" as referred to in the present invention means and includes polypeptides in general which are obtainable by the expression of microbial genes and contain the amino acid sequences of SEQ ID NOs:1 and 2 in whole or in part as a partial amino acid sequence. In general, the polypeptide according to the present invention contains an amino acid sequence revealed partly or completely: For example, those which contain the amino acid sequence of SEQ ID NO:3. In addition to a .beta.-fructofuranosidase activity, such a polypeptide catalyzes the fructofuranosyl transfer between appropriate fructofuranosyl donors and fructofuranosyl acceptors and has the following physicochemical properties:
(1) Molecular weight
About 44,000-54,000 daltons on SDS-PAGE;