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US 5,962,296 AGranted
Method for cloning and producing Thermomicrobium roseum DNA polymerase I in E. coli
AssigneeNew England Biolabs, Inc.
InventorsLaurence Ettwiller, Shuang-yong Xu
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Abstract
The present invention relates to isolating DNA coding for DNA polymerase I from Thermomicrobium roseum, expressing the T. roseum DNA polymerase I gene in E. coli and purifying the recombinant T. roseum DNA polymerase I from E. coli cell extract.
Claims6
A substantially pure thermostable DNA polymerase endogenous to Thermomicrobium roseum, ATCC No. 27502, wherein said polymerase has a molecular weight of about 103,000 daltons.
An isolated DNA which encodes a thermostable DNA polymerase with 3'-5' exonuclease activity from Thermomicrobium roseum ATCC No. 27502.
Dependencies are inferred from the claim text.