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OwnerSavas Anastassiadis, Alexander Aivasidis +1inventors
InventorsSavas Anastassiadis, Alexander Aivasidis, Christian Wandrey
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Strains of Aureobasidium pullulans (de bary) Arnaud can be used for the commercial production of gluconic acid by fermentation in aqueous liquid containing sugar, which in continuous culture from glucose form greater than or equal to 90% and greater than or equal to 90% molar selectivity. The process is conducted with an Fe and Mn optimized medium with a nitrogen-independent (N-independent) ion concentration. The iron (Fe) concentration with 3 g/l NH.sub.4 Cl is greater than or equal to 0.5 mM, and the manganese (Mn) concentration with 3 g/l NH.sub.4 Cl is greater than or equal to about 0.5 mM manganese (Mn). The pH is regulated between about 4.5 and about 8, in particular at 6.5-7, and the temperature between 24 and 32.degree. C., in particular at 29-30.degree. C. Particularly successful strains are Aureobasidium pullulans (de bary) Arnaud with the registration numbers DSM 7085, DSM 7086, DSM 7087, and DSM 7088.
A process for production of gluconic acid by fermentation in liquid growth medium comprising sugar, said process comprising;
A process for production of gluconic acid by fermentation in liquid media comprising sugar, said process comprising;
The process according to claimed 8, wherein said microorganism is retained with a filter in said medium as a biomass.
Dependencies are inferred from the claim text.
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Fungi ; Culture media therefor: fungi isolates
Containing a carboxyl group including Peroxycarboxylic acids: hydroxy-carboxylic acids
Preparation of compounds containing saccharide radicals: monosaccharides
Microorganisms ; Processes using microorganisms: fungi ; Processes using fungi
Term ended 28 May 2013 (estimated)
USPTO file history isn’t available for this patent, so only indexed dates are shown.
1. Field of the Invention
This invention relates to a process for the production of gluconic acid by fermentation in aqueuos fluid containing sugar. In addition, this invention also relates to the microorganisms suitable for producing gluconic acid by fermentation in aqueuos fluid containing sugar.
2. Background Information
The enclosed drawings are included to illustrate the dependence of gluconic acid production on individual parameters. In particular, continuous gluconic acid fermentation is examined, using Aureobasidium strain DSM 7085, which was found to have the highest activity. In the drawings and the accompanying description, the strain DSM 7085 may also be referred to as "isolate No. 70". In the drawings:
FIGS. 1 and 2 show the pH dependence;
FIGS. 3 to 5 show the influence of the dissolved oxygen concentration;
The subsequent tests were performed at about 30.degree. C. The organisms, however, are also capable of growth at lower temperatures, i.e. below about 30.degree. C.
For the isolation of the phyla, wildflowers from Julich, Dusseldorf, Heidelberg and Holland were comminuted and introduced into shaking flasks which contained a medium having the following concentration;
______________________________________ Glucose 100 g/l Yeast extract 3 g/l Citric acid 10 g/l Tween 40 2 drops/liter ______________________________________