Process for preparing L-tertiary-leucine and L-phosphinothricine by transamination
AssigneeHoechst Aktiengesellschaft
InventorsJohann Then, Klaus Bartsch, Hans-Matthias Deger, Susanne Grabley, Rudiger Marquardt
Life of this patent
Estimating the term…
Abstract
L-tertiary-Leucine and L-phosphinothricine are obtainable by transamination of the corresponding keto acids as a precursor in the presence of amino acids as amino group donors. The reaction is preferably carried out with microorganisms or their transaminases.
Claims18
An isolated microorganism capable of transaminating 3,3-dimethyl-2-oxo-butanoic acid, (3-carboxy-3-oxo-propyl)-methylphosphinic acid, or salts of these compounds in the presence of amino acids, wherein said microorganism is chosen from the group consisting of Alcaligenes faecaelis DSM 4115, Alcaligenes denitrificans DSM 4114, Pseudomonas paucimobilis DSM 4120, Pseudomonas spec. DSM 4119, Serattia plymuthica DSM 4116, Enterobacter agglomerans DSM 4122, Enterobacter spec. DSM 4121, Paracoccus denitrificans DSM 65, soil isolate DSM 4113, soil isolate DSM 4117, and soil isolate DSM 4118.
A process for preparing L-tertiary-leucine comprising incubating an .alpha.-keto acid selected from 3,3-dimethyl-2-oxo-butanoic acid and a salt of 3,3-dimethyl-2-oxo-butanoic acid in the presence of:
- (i) an L-amino acid as an amino group donor;
- and
- (ii) a microorganism strain capable of transaminating the .alpha.-keto acid, or transaminase enzyme of said microorganism strain.
A process for preparing L-phosphinothricine comprising incubating an .alpha.-keto acid selected from (3-carboxy-3-oxo-propyl)-methylphosphinic acid and a salt of (3-carboxy-3-oxo-propyl)-methylphosphinic acid in the presence of:
- (i) an L-amino acid as an amino group donor;
- and
- (ii) a microorganism strain capable of transaminating the .alpha.-keto acid, or transaminase enzyme of said microorganism strain.
Dependencies are inferred from the claim text.