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AssigneeNippon Shokubai Co., Ltd.
InventorsMasaharu Mukouyama, Satomi Komatsuzaki, Koichi Sakano
Estimating the term…
Enzyme-containing cells are immobilized on a water-insoluble support with a polymer represented by the following general formula (I): ##STR1## wherein Y is a direct bond or a divalent group represented by the following formula (II) ##STR2## R.sub.1 and R.sub.2 are each independently hydrogen atoms or organic residues, X.sup.- represents an anion, and n is an integer of 100 to 5000. The support may be in granular form such as granules of ion exchange resins or inorganic carriers, or in sheet form such as ion exchange films or alumina or silica sheets. Immobilization may be carried out by mixing cells with water and a quaternary salt of polyallylamine as the polymer, and sprinkling the resultant mixture onto the solid support and drying. L-aspartic acid or fumaric acid can be produced by contacting fumaric acid and ammonia, or ammonium fumarate, or maleic acid and ammonia, or ammonium maleate, with immobilized cells containing aspartase or maleate isomerase.
An immobilized biocatalyst comprising enzyme-containing cells which are immobilized on a water-insoluble solid support by a polymer represented by the following general formula:
Dependencies are inferred from the claim text.
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The carrier being a synthetic polymer: obtained by reactions only involving carbon-to-carbon unsaturated bonds
Enzymes or microbial cells immobilised on or in an organic carrier: entrapped within the carrier, e.g. gel or hollow fibres
Containing a carboxyl group including Peroxycarboxylic acids: polycarboxylic acids
Microorganisms or enzymes
Term ended 12 Feb 2018 (estimated)
USPTO file history isn’t available for this patent, so only indexed dates are shown.
1. Field of Invention
The present invention relates to a novel immobilized biocatalyst and its use.
2. Related Art
The present invention will now be explained in more detail by way of examples, although the invention is in no way to be limited thereby. The reaction products were analyzed by liquid chromatography.
A 5 L jar fermenter was loaded with 3 L of medium (pH 6.3) comprising a composition of 10 g of fumaric acid, 5 g of (NH.sub.4).sub.2 SO.sub.4, 1 g of KH.sub.2 PO.sub.4, 3 g of K.sub.2 HPO.sub.4, 0.5 g of MgSO.sub.4.7H.sub.2 O, 5.5 g of NaOH and 20 g of yeast extract in 1 L of distilled water, and Escherichia coli ATCC 11303 was inoculated thereto for aeration agitation culturing at 37.degree. C. Culturing was continued for twenty hours, and the cells were collected by centrifugation.
After preparing a mixture of 70 g of PAS-880 (product of Nitto Spinning, KK.) with the pH adjusted to near 7 with an alkali and 230 g of deionized water, the above-mentioned cells were dispersed therein. In a 6 L volume eggplant-shaped flask was placed 300 ml of an ion-exchange resin (Ambalite IRA-94S C1, average particle size: 0.5 mm, product of Organo, KK.) and 20 0.5-inch Teflon spheres, after which 1/6 of the previously obtained cell dispersion was added and subjected to vacuum drying for one hour with an evaporator at 30.degree. C. while rotating, to coat the ion-exchange resin with the cells. After this operation was repeated 6 times, the Teflon spheres were removed to obtain beads of immobilized biocatalyst.