Method for the enzymatic galactosylation of monosaccharides and oligosaccharides
AssigneeHoechst Aktiengesellschaft
InventorsBrigitte Horsch, Andreas Seiffert-Storiko, Rudiger Marquardt, Astrid Zervosen, Lothar Elling, Maria Regina Kula
Life of this patent
Estimating the term…
Abstract
The invention relates to an improved process for enzymically galactosylating monosaccharides and oligosaccharides, with in-situ regeneration of the nucleotide sugar (or of the nucleoside diphosphate sugar), in the presence of sucrose synthase, .beta.-1-4-galactosyl transferase and uridine diphosphate-glucose 4'-epimerase, (UDP-glucose 4'-epimerase), in which process the uridine diphosphate-glucose 4'-epimerase is reactivated with a ketosugar derivative.
Claims7
A process for enzymically galactosylating monosaccharides and oligosaccharides, with in-situ regeneration of a nucleotide sugar or of a nucleoside diphosphate sugar, in the presence of sucrose synthase, .beta.-1-4-galactosyl transferase and uridine diphosphate-glucose 4'-epimerase, wherein a deoxynucleoside diphosphate ketosugar, galactosone, allosone, glucosone or 6-deoxyglucosone is added to the reaction mixture as an activator of the uridine diphosphate-glucose 4'-epimerase.
A process for enzymically galactosylating monosaccharides and oligosaccharides, with in-situ regeneration of the nucleotide sugar, in the presence of sucrose synthase, .beta.-1-4-galactosyl transferase and uridine diphosphate-glucose 4'-epimerase, wherein deoxyuridine diphosphate-6-deoxy-D-xylohexulose is added to the reaction mixture as an activator of the uridine diphosphate-glucose 4'-epimerase.
A process for enzymically galactosylating monosaccharides and oligosaccharides, with in-situ regeneration of the nucleotide sugar, in the presence of sucrose synthase, .beta.-1-4-galactosyl transferase and uridine diphosphate-glucose 4'-epimerase, wherein deoxythymidine diphosphate-6-deoxy-D-xylohexulose is added to the reaction mixture as an activator of the uridine diphosphate-glucose 4'-epimerase.
Dependencies are inferred from the claim text.