Oxidative decarboxylation of peptides catalyzed by flavoprotein EpiD
AssigneeBoehringer Ingelheim International GmbH
InventorsThomas Kupke, Friedrich Gotz, Christoph Kempter, Gunther Jung
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Abstract
A method is described for oxidatively decarboxylating a peptide, comprising combining a peptide with EpiD, wherein the peptide comprises at its carboxy terminus the amino acid sequence X.sub.1 X.sub.2 X.sub.3 X.sub.4 X.sub.5 X.sub.6 C, wherein X.sub.1, X.sub.2, X.sub.3 and X.sub.4 are any one of the twenty common amino acids, X.sub.5 is Tyr, Val, Met, Leu, Ile, Phe or Trp, and X.sub.6 is Cys, Ala, Ser, Val, or Thr, whereby the oxidative decarboxylation of the peptide occurs.
Claims13
A method of oxidatively decarboxylating a peptide, comprising combining a peptide with EpiD, (SEQ ID NO:
- 24) said peptide comprising at its carboxy terminus the amino acid sequence X.sub.1 X.sub.2 X.sub.3 X.sub.4 X.sub.5 X.sub.6 C (SEQ ID NO:
- 1), wherein X.sub.1, X.sub.2, X.sub.3 and X.sub.4 are any one of the twenty common amino acids, X.sub.5 is Tyr, Val, Met, Phe, Ile, Leu or Trp, and X.sub.6 is Cys, Ala, Ser, Val, or Thr, with the proviso that the carboxy terminus of said peptide is not SFNSYCC (SEQ ID NO:
- 2), SFNSFCC (SEQ ID NO:
- 3), SFNSWCC (SEQ ID NO:
- 4) or SFNSYSC (SEQ ID NO:
- 5), whereby the oxidative decarboxylation of said peptide occurs.
A method of oxidatively decarboxylating a peptide, comprising combining a peptide with EpiD, said peptide comprising at its carboxy terminus the amino acid sequence SYCC (SEQ ID NO:
- 27), whereby the decarboxylation of said peptide occurs, with the proviso that the amino acid sequence immediately amino terminal to SYCC (SEQ ID NO:
- 27) is not SFN.
A bacterial host cell expressing
- (a) a recombinant DNA molecule encoding a peptide capable of being oxidatively decarboxylated by EpiD, with the proviso that said DNA molecule not encode a peptide comprising at its carboxy terminus the amino acid sequence SFNSYCC (SEQ ID NO:
- 2), SFNSFCC (SEQ ID NO:
- 3), SFNSWCC (SEQ ID NO:
- 4) or SFNSYSC (SEQ ID NO:
- 5);
- and
- (b) a recombinant DNA molecule encoding the protein EpiD having the amino acid sequence set forth in FIG. 9 (SEQ ID NO:
- 24).
A method of oxidatively decarboxylating a peptide, comprising combining a peptide with a fusion protein comprising EpiD, said peptide comprising at its carboxy terminus the amino acid sequence X.sub.1 X.sub.2 X.sub.3 X.sub.4 X.sub.5 X.sub.6 C (SEQ ID NO:
- 1), wherein X.sub.1, X.sub.2, X.sub.3 and X.sub.4 are any one of the twenty common amino acids, X.sub.5 is Tyr, Val, Met, Phe, Ile, Leu or Trp, and X.sub.6 is Cys, Ala, Ser, Val, or Thr, with the proviso that the carboxy terminus of said peptide is not SFNSYCC (SEO ID NO:
- 2), SFNSFCC (SEO ID NO:
- 3), SFNSWCC (SEQ ID NO:
- 4) or SFNSYSC (SEO ID NO:
- 5), whereby the oxidative decarboxylation of said peptide occurs.
Dependencies are inferred from the claim text.