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US 5,962,248 AGranted
Quantitative determination method for chloride ions
AssigneeKyowa Medex Co., Ltd.
InventorsToshio Tadano, Norihiko Kayahara, Jun Umemoto
Life of this patent
Estimating the term…
Abstract
Chloride ion is quantitatively determined in a sample by adding to the sample an enzyme having glucokinase activity to thereby eliminate glucose, admixing an .alpha.-amylase which has been deactivated by a chelating agent, and reacting the .alpha.-amylase with maltotetraose, maltohexaose or maltoheptaose.
Claims9
A method for quantitatively determining chloride ion in a sample, comprising the steps of:
- (1) admixing (i) an enzyme having a glucokinase activity, (ii) adenosine triphosphate and (iii) an aqueous medium comprising said sample;
- (2) reacting said sample with said enzyme to eliminate any glucose coexisting in said sample, to obtain mixture (A);
- (3) admixing mixture (A) with (i) an .alpha.-amylase which has been deactivated by a chelating agent and (ii) a chelating agent to deactivate the enzyme having a glucokinase activity, to obtain mixture (B);
- (4) reacting said .alpha.-amylase of mixture (B) with a naturally occurring oligosaccharide selected from the group consisting of maltotetraose, maltohexaose and maltoheptaose;
- (5) determining an amount of glucose produced in the reaction of step (4);
- and
- (6) correlating said amount of glucose with a quantity of chloride ion in said sample.
4 more elements
The method of claim 4, wherein said enzyme having a glucokinase activity is hexokinase type IV.
Dependencies are inferred from the claim text.