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US 5,962,244 AGranted
High-throughput in vitro assays for modulators of peptidyl transferase
AssigneeTularik Inc.
InventorsAnthony Simon Lynch, Michael Gregory Peterson, Binoj Joseph Matthew
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Abstract
High-throughput in vitro assays are provided for identifying modulators of peptidyl transferase. New solid-phase assays, related compositions, apparatus and integrated systems are provided.
Claims29
A method for determining peptidyl transferase activity, the method comprising:
- incubating a reaction mixture comprising a peptidlyl transferase, a peptidyl-tRNA analog which comprises a peptidyl moiety to which is attached an immobilizable tag, and an aminoacyl-tRNA analog under conditions suitable for transfer of the peptidyl moiety to said aminoacyl-tRNA analog;
- binding the immobilizable tag of the peptidyl moiety of said peptidyl-tRNA analog to a solid support;
- and
- detecting the presence of said aminoacyl-tRNA analog on said solid support as an indication of peptidyl transferase activity.
1 more element
A method for identifying a modulator of peptidyl transferase activity, the method comprising:
- incubating a reaction mixture comprising a peptidyl transferase, a peptidyl-tRNA analog which comprises a peptidyl moiety to which is attached an immobilizable tag, a aminoacyl-tRNA analog and a modulator under conditions suitable for transfer of the peptidyl moiety to said aminoacyl-tRNA analog;
- binding the immobilizable tag of the peptidyl moiety of said peptidyl-tRNA to a solid support;
- and
- detecting the presence of said aminoacyl-tRNA on said solid support as an indication of peptidyl transferase activity.
1 more element
Dependencies are inferred from the claim text.