Oligonucleotide constructs and methods for the generation of sequence signatures from nucleic acids
OwnerGustavo Caetano-Anollesinventor
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Abstract
Novel oligonucleotides for amplification and profiling of nucleic acid templates are disclosed. Enhancements of nucleic acid fingerprinting methods are disclosed.
Claims39
An oligonucleotide construct comprising a structure selected from the group consisting of (B.sub.x).sub.n (N).sub.m and (N).sub.m (B.sub.x).sub.n, wherein B is A, T, G, or C and wherein each B may be the same as or different than any other B in the construct so long as at least one B differs from another B, x is a whole number from 2 to 5, n is a whole number from 2 to 10, N is a degenerate base, and m is a whole number from 1 to 10.
An oligonucleotide mini-hairpin primer having a 3' single stranded end and a 5' end which primer comprises a double stranded stem of at least 2 complementary annealed base pairs, the double stranded stem extending to the 5' end of the primer and the other end of the stem extending as the single strand of at least 3 bases to the 3' end of the primer, and intermediate to the 5' and 3' ends of the stem, a single stranded loop of at least 3 bases.
An oligonucleotide primer having a 3' single stranded end and a 5' end which primer comprises a double stranded stem of 2 complementary annealed base pairs, the double stranded stem extending to the 5' end of the primer and the other end of the stem extending as the single strand of at least 3 bases to the 3' end of the primer, and intermediate to the 5' and 3' ends of the stem, a single stranded loop of 3 or 4 bases.
An oligonucleotide primer having a 3' single stranded end and a 5' end which primer comprises a double stranded stem of at least 2 complementary annealed base pairs, the double stranded stem extending to the 5' end of the primer and the other end of the stem extending as the single strand of at least 3 bases to the 3' end of the primer, and intermediate to the 5' and 3' ends of the stem, a single stranded loop.
An oligonucleotide primer having a 3' single stranded end and a 5' end which primer comprises a double stranded stem of at least 2 complementary annealed base pairs, the double stranded stem extending to the 5' end of the primer and the other end of the stem extending as the single strand of at least 3 bases to the 3' end of the primer, and intermediate to the 5' and 3' ends of the stem, a single stranded loop.
Dependencies are inferred from the claim text.