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AssigneeIGEN International, Inc.
InventorsJonathan K. Leland, Haresh P. Shah, John H. Kenten, Jack E. Goodman, George E. Lowke, Gary F. Blackburn, Richard J. Massey
Estimating the term…
The invention relates to methods, apparatus, reagents, and kits for performing a binding assay for an analyte of interest present in a sample based upon electrochemiluminescence at an electrode of interest. In the method, reagents and kits particles can be employed; for instance, for settling upon the electrode surface by gravity, centrifugation or magnetic attraction. The apparatus can include a magnet for generating a magnetic field in a region proximate the electrode.
A method for performing a binding assay for the detection or quantitation of an analyte of interest in a sample comprising the steps of:
A method for performing a binding assay for the detection or quantitation of an analyte of interest in a sample based upon measurement of electrochemiluminescence at an electrode surface comprising the steps:
A method for performing a binding assay for the detection or quantitation of an analyte of interest in a sample based upon measurement of electrochemiluminescence at an electrode surface comprising the steps:
A method for performing a binding assay for the detection or quantitation of an analyte of interest in a sample based upon measurement of electrochemiluminescence at an electrode surface comprising the steps:
A method for performing a binding assay for the detection or quantitation of an analyte of interest in a sample based upon measurement of electrochemiluminescence at an electrode surface comprising the steps:
A method for performing a binding assay for the detection or quantitation of an analyte of interest in a sample based upon measurement of electrochemiluminescence at an electrode surface comprising the steps:
A method for performing a binding assay for the detection or quantitation of an analyte of interest in a sample based upon measurement of electrochemiluminescence at an electrode surface comprising the steps:
An assay reagent for an assay based upon a binding reaction and the measurement of an electrochemiluminescent phenomenon comprising:
Dependencies are inferred from the claim text.
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From the US filing history. Foreign family members aren’t listed.
Electrically excited, e.g. electroluminescence: specially adapted for fluids , e.g. molten metal
Characterised by the detection means: nucleic acid detection involving sensors
Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated: chemiluminescence;…
Involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings: electrodes
Term ended 5 Oct 2016 (estimated)
USPTO file history isn’t available for this patent, so only indexed dates are shown.
This application relates generally to methods and apparatus for conducting binding assays, more particularly to those which measure the presence of an analyte of interest by measuring luminescence emitted by one or more labeled components of the assay system. More specifically, the invention relates to precise, reproducible, accurate homogeneous or heterogeneous specific binding assays of improved sensitivity in which the luminescent component is concentrated in the assay composition and collected on the detection system before being caused to electrochemiluminescence.
Numerous methods and systems have been developed for the detection and quantitation of analytes of interest in biochemical and biological substances. Methods and systems which are capable of measuring trace amounts of microorganisms, pharmaceuticals, hormones, viruses, antibodies, nucleic acids and other proteins are of great value to researchers and clinicians.
A very substantial body of art has been developed based upon the well known binding reactions, e.g., antigen-antibody reactions, nucleic acid hybridization techniques, and protein-ligand systems. The high degree of specificity in many biochemical and biological binding systems has led to many assay methods and systems of value in research and diagnostics. Typically, the existence of an analyte of interest is indicated by the presence or absence of an observable "label" attached to one or more of the binding materials. Of particular interest are labels which can be made to luminesce through photochemical, chemical, and electrochemical means. "Photoluminescence" is the process whereby a material is induced to luminesce when it absorbs electromagnetic radiation. Fluorescence and phosphorescence are types of photoluminescence. "Chemiluminescent" processes entail the creation of luminescent species by chemical transfer of energy. "Electrochemiluminescence" entails creation of luminescent species electrochemically.
FIG. 1 is a schematic drawing of a cell for performing the microparticulate-based nonseparation and separation assays of the invention.
FIG. 2 is a simplified diagram of a voltage control apparatus for use with the cell of FIG. 1.
FIG. 3 is a schematic representation of a direct incorporation PCR format using electrochemiluminescent labeled oligonucleotides and biotin electrochemiluminescent labeled oligonucleotides as primers.
In its broadest embodiment, the invention is in a method for performing a binding assay for an analyte of interest present in a sample. The steps include:
(a) forming a composition containing
(i) said sample