Vaccine and serum for endotoxin associated disease immunization and treatment, detoxified endotoxin, and bacterial mutant

A method for treating an animal diseased with a Gram negative bacterial disease, the steps of said method comprising:

• combining a killed suspension of non-O-carbohydrate side chain bacterial mutant ATCC 53000 as a bacterin, a detoxified lipopolysaccharide as the immune modulator having specific propensity for B-lymphocytes to cause rapid proliferation of antibody progenitor cells and their earlier occurrence, thus resulting in enhanced production of protective and neutralizing antibodies in a host's circulation against intact endotoxin and bacteria, and a protein and lipid binding carrier having high lipophilic and high proteinophilic affinity to ensure uniform component suspension and prolonged antigenic release as a vaccine;
• vaccinating a donor animal with said vaccine to produce antigen specific antibodies in response to said vaccine;
• collecting whole blood from said donor animal;
• forming clot serum containing said antigen specific antibodies from said whole blood;
• and
• introducing into said diseased animal sufficient serum to establish a protective level of said antigen specific antibodies.