Type I surface antigen associated with staphylococcus epidermidis
AssigneeNabi
InventorsAli Ibrahim Fattom, Walter W. Karakawa, D. Craig Wright
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Abstract
A process is disclosed for culturing clinical Staphylococcus epidermidis cells that reproducibly enables identification of a limited number of predominant serotypes. Two predominant serotypes common to most clinical cases of S. epidermidis have been identified and are denoted Type I and Type II. A particular polysaccharide surface antigen is associated with each of the Type I and Type II serotypes. The surface antigens can be used to provide active and passive immunization against S. epidermidis infection and to produce a hyperimmune immunoglobulin or antibodies for treatment of S. epidermidis infection.
Claims12
A hyperimmune globulin containing antibodies directed against S. epidermidis polysaccharide antigen that is obtained by a process comprising:
- growing cells of a Type I isolate of S. epidermidis that agglutinates antisera to ATCC 55254;
- extracting polysaccharide antigen from said cells to produce a crude extract of Type I polysaccharide antigen;
- purifying said crude extract to produce purified antigen that contains less than 1% protein;
- loading said purified antigen on a separatory column and eluting it with a NaCl gradient ranging from 0-0.3 M;
- identifying fractions containing Type I polysaccharide antigen using antibodies specific to a Type I isolate, and
- immunizing a plasma donor with said Type I polysaccharide antigen in order to obtain a hyperimmune globulin.
Dependencies are inferred from the claim text.