Simple micro method for concentration and desalting utilizing a hollow fiber, with special reference to capillary electrophoresis
AssigneeBio-Rad Laboratories, Inc.
InventorVilhelm Einar Stellan Hjerten
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Abstract
A dilute aqueous solution in microvolume quantity containing water soluble substances is concentrated by placing the solution in a length of hollow fiber whose wall contains pores sized to selectively pass water, while replenishing the fiber volume with additional solution at a rate approximately equal to the rate that the water passes through the fiber wall. A zone of concentrated solution is formed within the fiber, and the concentrated solution is then recoverable from the zone.
Claims28
Apparatus for the concentration of microvolumes of a dilute aqueous solution of water soluble substances comprising a discrete strand of hollow fiber adapted to receive an initial amount of said solution, the walls of the fiber containing pores sized to pass water therethrough, and means associated with said strand for replenishing the volume of liquid within the strand by drawing additional quantities of said solution into said fiber solely due to the passage of water through said wall at a rate approximately equal to the rate of water passing through said wall.
The method of concentrating microvolumes of a dilute aqueous solution of water soluble substances comprising at least partially filling a discrete strand of hollow fiber with said solution, the walls of said fiber containing pores sized to pass water therethrough while preventing passage of said biological molecules, establishing fluid communication between the interior of said strand and a reservoir containing said solution of water soluble substances, allowing additional amounts of solution to be drawn into the fiber solely due to the passage of water through said wall at a rate approximately equal to the rate of water passing through said wall, thereby developing a zone of concentrated solution within said fiber, and recovering the concentrated solution from said zone.
Dependencies are inferred from the claim text.