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US 5,958,775 AGranted
Composition and method for targeted integration into cells
AssigneeThomas Jefferson University
InventorsEric Wickstrom, Stephen Cleaver
Life of this patent
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Abstract
A vector containing a selected nucleic acid sequence interposed between the terminal ends of a Tn7 transposon and a method for site specific integration of genetic material into a chromosome using those vectors are provided.
Claims4
A method for site specific integration of genetic material into a chromosome in a host cell at a site analogous to the attTn7 site of the E. coli chromosome, the method comprising the steps of:
- (a) constructing a vector which contains each terminal end of a Tn7 transposon and a selected nucleic acid sequence interposed between said terminal ends;
- (b) binding said vector to TnsB;
- (c) complexing the vector and bound TnsB of step (b) with TnsA, TnsC, TnsD;
- (d) introducing the complex of step (c) to a host cell;
- and
- (e) growing said host cells for sufficient generations and under suitable conditions for the inserted genetic to integrate into the chromosome of the host cell.
3 more elements
A complex comprising a vector which contains each terminal end of a Tn7 transposon and a selected nucleic acid sequence interposed between said terminal ends;
- and TnsA, TnsB, TnsC and TnsD complexed to said vector.
Dependencies are inferred from the claim text.