Branch migration of nucleotides
AssigneeEnzo Therapeutics, Inc.
InventorsJames G. Wetmur, Robin S. Quartin, Dean L. Engelhardt
Life of this patent
Estimating the term…
Abstract
Procedures and compositions for forming and stabilizing non stable branch migrated oligo- and polydeoxynucleotides utilizing a displacer which is either single stranded or partially double stranded and hybridized to a linker strand. The displacer may contain one or more modified nucleotides.
Claims116
In a method of forming a non stable complex between one strand of a recipient polydeoxynucleotide sequence and a displacer sequence of single stranded DNA where the displacer sequence is at least partially complementary to such strand of a recipient polydeoxynucleotide sequence, the improvement comprising stabilizing the complex.
An oligo- or polydeoxynucleotide displacer-linker duplex which is capable of initiating branch migration at the end of a recipient polydeoxynucleotide duplex without the prior formation of a stable hybrid with such recipient polydeoxynucleotide duplex, which displacer-linker duplex comprises two strands;
- a. a displacer strand of which a portion comprises nucleotides complementary to one strand of a recipient polydeoxynucleotide duplex and a portion which comprises a sequence complementary to and hybridized with a linker strand, and
- b. a linker strand complementary to and hybridized with the displacer strand.
A method of modifying a recipient polydeoxynucleotide duplex by contacting such recipient polydeoxynucleotide duplex with a displacer-linker duplex under conditions that permit the formation of a hybrid polydeoxynucleotide duplex, wherein
- the displacer-linker duplex comprises two strands;
- 1. a displacer strand of which a portion comprises nucleotides complementary to one strand of a recipient polydeoxynucleotide duplex and a portion which comprises a sequence complementary to and hybridized with a linker strand, and
- 2. a linker strand complementary to and hybridized with the displacer strand.
A method of labelling an artificially constructed nucleic acid hybrid of a naturally occurring recipient polydeoxynucleotide duplex hybridized to a displacer-linker duplex which is capable of initiating branch migration at the end of the recipient polydeoxynucleotide duplex without the prior formation of a stable hybrid with such recipient polydeoxynucleotide duplex, which displacer-linker duplex comprises two strands:
- 1. a displacer strand of which a portion comprises nucleotides complementary to one strand of a recipient polydeoxynucleotide duplex and a portion which comprises a sequence complementary to and hybridized with a linker strand, and
- 2. a linker strand complementary to and hybridized with the displacer strand,
- which method of labelling comprises modifying the displacer-linker duplex to incorporate therein a modification which permits detection of the artificially constructed nucleic acid hybrid.
An oligo- or polydeoxynucleotide displacer which is capable of binding to a recipient polydeoxynucleotide duplex which displacer comprises
- 1) a first sequence which is capable of initiating triple helix formation, and which comprises
- a) at least six consecutive pyrimidine bases or
- b) at least seven bases where at least six of the bases are pyrimidine bases and the seventh base is guanine, and
- 2) a second sequence proximate to such first sequence which is complementary to and runs antiparallel to the second strand of the recipient duplex and which is capable of initiating branch migration proximate to the triple helix.
Dependencies are inferred from the claim text.