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AssigneeGen-Probe Incorporated
InventorsJames John Hogan, Jo Ann Kop, Sherrol Hoffa McDonough
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A method for preparing probes, as well as several probes for use in qualitative or quantitative hybridization assays are disclosed. The method comprises constructing an oligonucleotide that is sufficiently complementary to hybridize to a region of rRNA selected to be unique to a non-viral organism or group of non-viral organisms sought to be detected, said region of rRNA being selected by comparing one or more variable region rRNA sequences of said non-viral organism or group of non-viral organisms with one or more variable region rRNA sequences from one or more non-viral organisms sought to be distinguished. Hybridization assay probes for Mycobacterium avium, Mycobacterium intracellulare, the Mycobacterium tuberculosis-complex bacteria, Mycoplasma pneumoniae, Legionella, Salmonella, Chlamydia trachomatis, Campylobacter, Proteus mirabilis, Enterococcus, Enterobacter cloacae, E. coli, Pseudomonas group I, Neisseria gonorrhoeae, bacteria, and fungi also are disclosed.
A probe comprising an oligonucleotide 15 to 100 nucleotides in length able to hybridize to an Enterobacter cloacae nucleic acid target region to form a detectable target:probe duplex under high stringency hybridization assay conditions, said target region corresponding to, or fully complementary to and of the same length as a nucleic acid corresponding to, bases 305-340 of E. coli 23S rRNA;
A method for determining whether Enterobacter cloacae may be present in a sample comprising the steps of:
Dependencies are inferred from the claim text.