Probes for papillomaviruses and an in vitro diagnostic procedure for papilloma infections
AssigneesInstitut Pasteur, Institut Nationale de la Sante et de la Recherche Medicale
InventorsSylvie Beaudenon, Dina Kremsdorf, Odile Croissant, Gerard Orth
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Abstract
The invention relates to papillomaviruses, particularly to DNA-HPVs isolated from the papillomaviruses IP5 and IP6, the restriction maps of papillomaviruses IP5 and IP6, and also to probes containing these DNA-HPVs or fragments obtained from them. The invention relates, in addition, to "kits" containing distinct groups of probes, containing one of these DNA-HPVs or DNA-HPV fragments, as well as a procedure for the detection and identification of papillomaviruses which makes use of these different probes.
Claims25
A purified DNA of human papillomaviruses, which is a gene selected from the group consisting of E1, E6-E7, L1, and L2,
- wherein said human papillomavirus is selected from the group consisting of HPV-IP5 and HPV-IP6.
A purified DNA, which hybridizes to the DNA of a human papillomavirus (HPV) selected from the group consisting of HPV-IP5 and HPV-IP6.
A probe for the detection of human papillomavirus (HPV) DNA, which hybridizes under stringent conditions with the genome of a human papillomavirus selected from the group consisting of HPV-IP5 and HPV-IP6.
A kit comprising a plurality of different probes for detecting human papillomavirus (HPV) DNA, wherein said kit contains at least one probe selected from each of the following groups a-k:
- a) DNA of HPV2d;
- b) DNA of HPV10b, 28 and 29;
- c) DNA of HPV17, 24;
- d) DNA of HPV14, 15, 17, 19, 20, 21, 22, 23 and IP4;
- e) DNA of HPV15 and 17;
- f) DNA of HPV24;
- g) DNA of HPV14, 32 and IP4;
- h) DNA of HPV31;
- i) DNA of HPV32;
- j) DNA of HPV16, 18, IP2 and IP5;
- and
- k) DNA of HPV6, 11 and IP6,
- wherein each of the probes in said kit is of a different HPV type from the other probes in said kit.
A kit for the in vitro detection of infection by human papillomavirus, said kit comprising DNA probes of human papillomavirus types in a mixture selected from the following groups a-k:
- a) DNA of HPV2d;
- b) DNA of HPV10b, 28 and 29;
- c) DNA of HPV17, 24;
- d) DNA of HPV14, 15, 17, 19, 20, 21, 22, 23 and IP4;
- e) DNA of HPV15 and 17;
- f) DNA of HPV24;
- g) DNA of HPV14, 32 and IP4;
- h) DNA of HPV31;
- i) DNA of HPV32;
- j) DNA of HPV16, 18, IP2 and IP5;
- and
- k) DNA of HPV6, 11 and IP6.
A purified recombinant DNA molecule produced by the process comprising cloning a vector in a host cell, wherein said vector comprises human papillomavirus DNA selected from the HPV group consisting of HPV IP5 and HPV IP6.
A purified recombinant DNA molecule produced by a process comprising cloning a vector in a host cell, wherein said vector comprises human papillomavirus IP5 DNA.
A cloned recombinant DNA molecule produced by a process comprising cloning a vector in a host cell, wherein said vector comprises human papillomavirus IP6 DNA.
A kit for the in vitro detection of infection by human papillomavirus, said kit comprises eleven DNA probes of human papillomavirus types in a mixture, wherein said DNA probes are from the human papillomaviruses selected from the following groups a-k:
- a) HPV1, 2d and 4;
- b) HPV3, 10a, 10b, 28, and 29;
- c) HPV5, 17a, and 24;
- d) HPV5, 8, 12, 14a, 14b, 19, 20, 21, 22, 23, and IP4;
- e) HPV9, 15, 17a, and 17b;
- f) HPV24;
- g) HPV5, 8, 14b, 32, and IP4;
- h) HPV13 and 31;
- i) HPV32 and 1P4;
- j) HPV16, 18, IP2, and IP5;
- and
- k) HPV6, 11, and IP6.
Dependencies are inferred from the claim text.