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US 5,958,672 AGranted
Protein activity screening of clones having DNA from uncultivated microorganisms
AssigneeDiversa Corporation
InventorJay M. Short
Life of this patent
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Abstract
Disclosed is a process of screening clones having DNA from an uncultivated microorganism for a specified protein, e.g. enzyme, activity by screening for a specified protein, e.g. enzyme, activity in a library of clones prepared by (i) recovering DNA from a DNA population derived from at least one uncultivated microorganism; and (ii) transforming a host with recovered DNA to produce a library of clones which is screened for the specified protein, e.g. enzyme, activity.
Claims15
A method for identifying a protein activity of interest comprising:
- culturing a gene expression library comprising a pool of expression constructs, each expression construct comprising a vector containing one or more cDNA or genomic DNA fragments, wherein the cDNA or genomic DNA fragments in the pool of expression constructs are derived from a plurality of species of donor organisms, and wherein the cDNA or genomic DNA fragments are each operably-associated with one or more regulatory regions that drives expression of genes encoded by the cDNA or genomic DNA fragments in an appropriate host organism;
- and
- detecting the protein activity encoded by the cDNA or genomic DNA fragments.
A method for identifying a protein activity of interest comprising:
- culturing a gene expression library, comprising a pool of expression constructs, each expression construct comprising a vector containing one or more cDNA or genomic DNA fragments, wherein the cDNA or genomic DNA fragments in the pool of expression constructs are derived from a plurality of species of donor microorganisms, and wherein the cDNA or genomic DNA fragments are each operably-associated with one or more regulatory regions that drives expression of genes encoded by the cDNA or genomic DNA fragments in an appropriate host organism;
- and
- detecting the protein activity encoded by the cDNA or genomic DNA fragments.
Dependencies are inferred from the claim text.