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AssigneeFoschungszentrum Julich GmbH
InventorsMarion Thust, Michael J. Schoning, Joachim Vetter, Ulrich B. Kaupp, Peter Kordos, Hans Luth
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Biomaterial such as an enzyme, cell, antigen or antibody is immobilized by covalent bonding to a substrate having an Si.sub.3 N.sub.4 surface containing Si--NH.sub.2 groups that provide reactive NH.sub.2 groups. A heterobifunctional cross-linking agent having an NH.sub.2 -reactive group and a biomaterial-reactive group is reacted with the NH.sub.2 groups of the surface and then with the biomaterial or the heterobifunctional cross-linking agent is reacted with the biomaterial and then with the NH.sub.2 groups of the surface. The Si.sub.3 N.sub.4 surface containing Si--NH.sub.2 groups can be formed by precipitating on a substrate a 10-1000 nm thick Si.sub.3 N.sub.4 layer from a SiH.sub.4 /NH.sub.3 mixture, and providing reactive NH.sub.2 groups on the surface of the layer by subjecting the surface to hydrolyzing cleaning.
A method for the immobilization of biomaterial on a substrate with an Si.sub.3 N.sub.4 surface to which the biomaterial is covalently bonded by means of a hetero-bifunctional cross-linking agent, said method comprising the steps of:
A Method for the immobilization of biomaterial on a substrate with an Si.sub.3 N.sub.4 surface to which the biomaterial is covalently bonded by means of a hetero-bifunctional cross-linking agent, said method comprising the steps of providing a substrate with a Si.sub.3 N.sub.4 surface having Si--NH.sub.2 groups to provide reactive NH.sub.2 groups, providing a hetero-bifunctional cross-linking agent having an NH.sub.2 -reactive group selected from the group consisting of aldehyde, ester, halogenide, epoxide, imine and isocyanate, and a biomaterial-reactive group, reacting a biomaterial with the biomaterial-reactive group of the cross-linking agent to covalently bond the biomaterial to the cross-linking agent, and reacting the NH.sub.2 -reactive group of the cross-linking agent with the NH.sub.2 groups of the Si.sub.3 N.sub.4 surface to covalently bond the cross-linking agent to the surface and mobilize the biomaterial on the Si.sub.3 N.sub.4 surface of the substrate.
Dependencies are inferred from the claim text.