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US 5,955,318 AGranted
Reagents and methods useful for controlling the translation of hepatitis GBV proteins
AssigneeAbbott Laboratories
InventorsJohn N. Simons, Suresh M. Desai, Isa K. Mushahwar
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Abstract
Reagents and composition for controlling the translation of hepatitis GB virus (HGBV)-A, -B or -C peptides from viral nucleic acid. These reagents and methods comprise control elements of the 5' NTR region of the HGBV-A, -B, or -C viral genome.
Claims5
A method for altering the translation of a hepatitis GB virus (HGBV) nucleic acid(s) to an HGBV protein(s) in vitro, comprising:
- (a) hybridizing a non-naturally occurring nucleic acid sequence to a sequence of the sense strand within the 5' non-translated region (NTR) of HGBV-A, HGBV-B or HGBV-C wherein said nucleic acid sequence has at least 10 nucleotides and is complementary to said 5' NTR region of HGBV-A, HGBV-B or HGBV-C;
- and
- (b) translating said sequence of said sense strand.
A method of enhancing the translation of a first nucleic acid in vitro comprising:
- (a) forming a combined nucleic acid by operably linking said first nucleic acid with a second nucleic acid having a sequence of the HGBV-A, HGBV-B or HGBV-C 5' NTR region, wherein said second nucleic acid controls translation of said first nucleic acid;
- and
- (b) translating said combined nucleic acid.
Dependencies are inferred from the claim text.