Nucleic acid molecules encoding cytochrome P450-type proteins involved in the brassinosteroid synthesis in plants
AssigneeMax-Planck-Gesellschaft zur Forderung der Wissenschaften e.V.
InventorsCsaba Koncz, Jaideep Mathur, Miklos Szekeres, Thomas Altmann
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Abstract
The invention describes nucleic acid molecules encoding cytochrome P450-t proteins involved in the brassionosteroid synthesis in plants, transgenic plant cells and plants containing such nucleic acid molecules as well as processes for the identification of other proteins involved in brassinosteroid synthesis and processes for the identification of substances acting as brassinosteroids or as brassinosteroid inhibitors in plants.
Claims22
An isolated nucleic acid molecule encoding a protein having the enzymatic activity of a cytochrome P450-type hydroxylase or encoding a biologically active fragment of such a protein, selected from the group consisting of:
- (a) nucleic acid molecules coding for a polypeptide having the amino acid sequence given in SEQ ID NO:
- 2;
- (b) nucleic acid molecules comprising the coding region of the nucleotide sequence given in SEQ ID NO:
- 1;
- (c) the nucleic acid molecule of SEQ ID NO:
- 3;
- (d) nucleic acid molecules hybridizing under stringent conditions to a nucleic acid molecule of (a), (b) or (c);
- and
- (e) nucleic acid molecules which are degenerate to the nucleic acid molecules of any one of (a) to (d).
A transgenic plant cell, comprising:
- a nucleic acid molecule selected from the group consisting of;
- (a) nucleic acid molecules coding for a polypeptide having the amino acid sequence given in SEQ ID NO:
- 2;
- nucleic acid molecules comprising the coding region of the nucleotide sequence given in SEQ ID NO:
- 1;
- (c) the nucleic acid molecule of SEQ ID NO:
- 3;
- (d) nucleic acid molecules hybridizing under stringent conditions to a nucleic acid molecule of (a), (b) or (c);
- and
- (e) nucleic acid molecules which are degenerate to the nucleic acid molecules of any one of (a) to (d),
- which has been integrated in a stable manner into the genome of said plant cell, wherein the expression of the nucleic acid molecule leads to a reduction in the synthesis in the cells of the polypeptide having the amino acid sequence given in SEQ ID NO:
- 2.
Dependencies are inferred from the claim text.