Topiramate immunoassay
AssigneeOxis International, Inc.
InventorsKenneth J. Stenglein, Daniel B. Cawley, Bruce E. Maryanoff, Kirk L. Sorgi
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Abstract
The present invention provides a topiramate immunoassay and reagents for use in the immunoassay. In particular, topiramate is derivatized at the sulfamate moiety or the 9-carbon or 10-carbon methyl group of topiramate to add a label bound directly or through a linking group for use as a tracer (competitive analyte analog) or to add a linking group bound to a carrier for use as an immunogen to induce anti-topiramate antibodies. Immunoassay methods and kits are also provided.
Claims37
A topiramate analog of a formula selected from the group consisting of a compound of the formula ##STR24## wherein one of R.sub.1 and R.sub.2 is hydrogen and the other is R-Y, R is a linking group of the formula (CH.sub.2).sub.n CO-- wherein n=1-9, and Y is an immunogenic carrier or a detectible label having a terminal amine group with the proviso that when R.sub.1 is hydrogen, then X is hydrogen, and with the further proviso that when R.sub.1 is R-Y, then X is hydrogen or an alkyl group;
- and the corresponding 10-carbon R.sub.2 derivative.
An anti-topiramate antibody.
An immunoassay kit for assaying topiramate comprising:
- a. an anti-topiramate antibody;
- and
- b. a topiramate analog of the formula selected from the group consisting of:
- ##STR25## wherein one of R.sub.1 and R.sub.2 is H and the other is R-Y, R is a linking group of the formula (CH.sub.2).sub.n CO-- wherein n=1-9, and Y is a detectable label, when R.sub.1 is H, X is H, when R.sub.1 is not H, X is H or an alkyl group.
A method for assaying topiramate in a sample comprising the steps of:
- (a) combining the sample with a topiramate analog of a formula selected from the group consisting of a compound of the formula ##STR26## wherein one of R.sub.1 and R.sub.2 is hydrogen and the other is R-Y, R is a linking group of the formula (CH.sub.2).sub.n CO-- wherein n=1-9, and Y is a detectible label having a terminal amine group, with the proviso that when R.sub.1 is hydrogen, then X is hydrogen, and with the further proviso that when R.sub.1 is R-Y, then X is hydrogen or an alkyl group;
- the corresponding 10-carbon R.sub.2 derivative;
- and anti-topiramate antibodies;
- (b) determining the amount of antibody bound to the topiramate analog as an indication of the amount of topiramate in the sample.
A topiramate analog of the formula:
- ##STR27## wherein R.sub.2 is hydrogen, R.sub.1 is R-Y, R is a linking group of the formula (CH.sub.2).sub.n CO-- wherein n=1 to 9, and Y is an immunogenic carrier or a detectable label having a terminal amine group;
- and X is hydrogen or an alkyl group.
Dependencies are inferred from the claim text.