Method of binding two or more DNA double helices and products formed
AssigneeSimon Fraser University
InventorsDipankar Sen, Eduard Antoni Venczel
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Abstract
This invention relates to a nucleic acid complex having double-stranded sections with a domain of guanine nucleotides. The domain comprises of a pair of substantially uninterrupted guanine sequences which bond together. This domain can interact with other similar domains such that two nucleic acid complexes with these domains have the ability to bind together to form DNA superstructures. The present invention also relates to a method of forming a nucleic acid superstructure using the engineered nucleic acid complexes.
Claims58
A double-stranded nucleic acid complex, comprising
- a) a plurality of individual strands of nucleic acids, each strand bound antiparallel to another of said strands, along substantially their entire length, to form paired strands in the form of double helices;
- b) at least one Watson-Crick domain on said paired strands, said Watson-Crick domain being a region of a least one bound base-pair of said paired strands wherein within each said Watson-Crick domain, each Watson-Crick base on a first strand of said paired strands is bound to a complementary Watson-Crick base on a second strand of said paired strands;
- and
- c) at least one guanine domain on said paired strands, said guanine domain being a region of bound guanine pairs of said paired strands wherein within each said guanine domain, a guanine sequence of substantially contiguous guanines on said first strand of said paired strands, is bound to a guanine sequence of substantially contiguous guanines on said second strand of said paired strands to form said guanine domain.
A double-stranded nucleic acid complex, comprising
- a) at least one individual strand of nucleic acid, wherein said strand is folded to form three or more arms of double helices, each of said arms is formed by binding two portions of said strand oriented as to be antiparallel, and
- b) at least one Watson-Crick domain on each of said arms of said strand wherein each Watson-Crick base on a portion of said strand of said arm binds with another complementary Watson-Crick base on another portion of said strand of said arm, and
- c) at least one guanine domain of substantially contiguous guanines on each of said arms of said strand wherein guanines on a portion of said strand of said arm bind with guanines on another portion of said strand of said arm.
Dependencies are inferred from the claim text.