Neural thread protein gene expression and detection of Alzheimer's disease
AssigneeThe General Hospital Corporation
InventorsSuzanne de la Monte, Jack R. Wands
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Abstract
The present invention is directed to recombinant hosts expressing novel proteins associated with Alzheimer's Disease, neuroectodermal tumors, malignant astrocytomas, and glioblastomas. This invention is specifically directed to the recombinant hosts and vectors which contain the genes coding for the neuronal thread proteins. This invention is also directed to substantially pure neural thread protein, immunodiagnostic and molecular diagnostic methods to detect the presence of neural thread proteins, and the use of nucleic acid sequences which code for neural thread proteins in gene therapy.
Claims34
A Neural Thread Protein (NTP) substantially free of any natural impurities, wherein said protein is specifically recognized by monoclonal antibody #2 on deposit with the American Type Culture Collection, Manassas, Va., under accession number HB-12546 or monoclonal antibody #5 on deposit with the American Type Culture Collection, Manassas, Va., under accession number HB-12545.
The Neural Thread Protein (NTP) substantially free of any natural impurities and coded for by the G2-2 Pst1 DNA molecule present in the DH5 E. coli cells that are on deposit at the American Type Culture Collection, Manassas, Va., under accession no. 69257.
A Neural Thread Protein (NTP) substantially free of any natural impurities and coded for by the G5d-Pst1 DNA molecule present in the DH5 E. coli cells that are on deposit at the American Type Culture Collection, Manassas, Va., under accession no. 69258.
A Neural Thread Protein (NTP) substantially free of any natural impurities and coded for by the 1-9a DNA molecule present in the E. coli cells that are on deposit at the American Type Culture Collection, Manassas, Va., under accession no. 69259.
A Neural Thread Protein (NTP) substantially free of any natural impurities and coded for by the AD3-4 DNA molecule present in the DH1 E. coli cells that are on deposit at the American Type Culture Collection, Manassas, Va., under accession no. 69260.
A Neural Thread Protein (NTP) substantially free of any natural impurities and coded for by the HB4 DNA molecule present in the E. coli cells that are on deposit at the American Type Culture Collection, Manassas, Va., under accession no. 69261.
A Neural Thread Protein (NTP) substantially free of any natural impurities and coded for by the AD10-7 DNA molecule present in the DH1 E. coli cells that are on deposit at the American Type Culture Collection, Manassas, Va., under accession no. 69262.
A Neural Thread Protein (NTP) substantially free of any natural impurities and coded for by the AD2-2 DNA molecule present in the DH1 E. coli cells that are on deposit at the American Type Culture Collection, Manassas, Va., under accession no. 69263.
A Neural Thread Protein (NTP) substantially free of any natural impurities and coded for by the G5d-1 Pst1-EcoR1 DNA molecule present in the DH5 E. coli cells that are on deposit at the American Type Culture Collection, Manassas, Va., under accession no. 69264.
A Neural Thread Protein (NTP) substantially free of any natural impurities and coded for by the G2-2 Pst1-EcoR1 DNA molecule present in the DH5 E. coli cells that are on deposit at the American Type Culture Collection, Manassas, Va., under accession no. 69265.
A Neural Thread Protein (NTP) substantially free of any natural impurities, wherein said protein is specifically recognized by monoclonal antibody #2 on deposit with the American Type Culture Collection, Manassas, Va., under accession number HB-12546 or monoclonal antibody #5 on deposit with the American Type Culture Collection, Manassas, Va., under accession number HB-12545, wherein said NTP is obtained by a process comprising:
- (a) culturing a recombinant host comprising a gene coding for said NTP;
- and
- (b) isolating said NTP from said host.
Dependencies are inferred from the claim text.