Chromatographic resins and methods for using same
AssigneeMassey University
InventorsSimon C. Burton, Neill Ward Haggarty, David R. K. Harding, Nathaniel Todd Becker, Ben A. Bulthuis, Landon M. Steele
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Abstract
Disclosed are rationally designed mixed mode resins which are useful in recovering a target compound from an aqueous solution and methods for use of such resins. The resins described herein have a hydrophobic character at the pH of binding of the target compound and a hydrophilic and/or electrostatic character at the pH of desorption of the target compound from the resin and are specifically designed to bind the target compound from an aqueous solution at both a low and high ionic strength.
Claims3
A resin having ionizable functional groups pendant to a solid support matrix which resin comprise:
- a solid support matrix;
- and
- an ionizable ligand which comprises an ionizable functionality and a spacer arm which covalently attaches the ionizable functionality to the solid support matrix wherein said ionizable functionality is derived from a compound selected from the group consisting of 4-(aminomethyl)pyridine, 3-(aminomethyl)pyridine, 2-(aminomethyl)pyridine, 1-(3-aminopropyl)-imidazole, 2-(aminomethyl)-benzimidazole, 4-(3-aminopropyl)morpholine, halotryamines having from 1 to 2 halo groups selected from the group consisting of fluoro, chloro, bromo and iodo, 4-aminobutyric acid, (1S, 2S)-(+)-2-amino-1-phenyl-1,3-propanediol, phenylethylamine, phenylalaninol, 4-(2-aminoethyl)benzenesulfonamide, DL-.beta.-hydroxyphenylethylamine, thiosalicylic acid, 2-mercaptobenzimidazole, 4-mercaptoethylpyridine, 2-mercaptoethylpyridine, 4-mercaptobutyric acid, 5-mercaptovaleric acid, 6-mercaptohexanoic acid, 4-hydroxythiophenol, 4-hydroxybenzoic acid, 4-hydroxyphenylacetic acid, 3-chlorohydroxyphenylacetic acid, 3,5-dichlorosalicylic acid, 4-hydroxy-3-nitrobenzoic acid, 3-nitrotyrosine, halotyrosines having from 1 to 2 halo groups selected from the group consisting of fluoro, chloro, bromo and iodo, and para-hydroxybenzoic acid hydrazide.
A resin having ionizable functional groups pendant to a solid support matrix which resin comprises:
- a solid support matrix;
- and
- ionizable ligand pendant to said support at a density greater than the smaller of either at least about 150 .mu.mol per milliliter of resin or 1 mmol per gram dry weight of resin which comprises an ionizable functionality and a spacer arm which covalently attaches the ionizable functionality to the solid support matrix wherein said ionizable functionality is derived from either 2-mercapto-1-methylimidizole or (-)-phenylpropanolamine.
Dependencies are inferred from the claim text.