Prorenin antibody and renin-active substance containing the same
AssigneesTokiwa Chemical Industries Co., Ltd., Yuichi Ishida
InventorsKazuo Murakami, Yukio Nakamura, Fumiaki Suzuki, Yuichi Ishida, Yasuhiko Hatano
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Abstract
Provided by the present invention are a human prorenin profragment N-terminated peptide antibody capable of specifically recognizing a peptide containing at least 15 amino acid residues from the first leucine residue to the 15th arginine residue in the N-terminated peptide of the human prorenin profragment and a complex of the same with human prorenin. The antibody can be used as a prorenin assay reagent. The human prorenin profragment N-terminated peptide antibody is useful as a reagent for the assay of human prorenin in blood.
Claims3
An isolated and purified antibody to human prorenin profragment N-terminated peptide, said antibody activating prorenin by specifically recognizing a peptide consisting of 15 amino acid residues from the first leucine residue to the 15th arginine residue in the N-terminated peptide of the human prorenin profragment.
A method for the assay of human prorenin in blood which comprises the steps of:
- a) reacting human prorenin in blood with an antibody to a human prorenin profragment N-terminated peptide, said antibody activating prorenin by specifically recognizing a peptide consisting of 15 amino acid residues from the first leucine residue to the 15th arginine residue in the N-terminated peptide of the human prorenin fragment, to form a complex which is a renin active substance;
- b) adding angiotensinogen as a renin substrate to the renin-active substance to form angiotensin I;
- and
- c) determining the angiotensin I.
Dependencies are inferred from the claim text.