Methods and compositions for multiple gene transfer into bone cells
AssigneeThe Regent of the University of Michigan
InventorsJeffrey Bonadio, Steven A. Goldstein
Life of this patent
Estimating the term…
Abstract
Disclosed are methods, compositions, kits and devices for use in transferring nucleic acids into bone cells in situ and/or for stimulating bone progenitor cells. Type II collagen and, particularly, osteotropic genes, are shown to stimulate bone progenitor cells and to promote bone growth, repair and regeneration in vivo. Gene transfer protocols are disclosed for use in transferring various nucleic acid materials into bone, as may be used in treating various bone-related diseases and defects including fractures, osteoporosis, osteogenesis imperfecta and in connection with bone implants.
Claims130
A method for transferring nucleic acid segments into bone progenitor cells located within a bone progenitor tissue site of an animal, comprising contacting said tissue site with a composition comprising two or more nucleic acid segments and a structural bone-compatible matrix, so as to transfer said two or more nucleic acid segments into said cells, wherein said cells express transcriptional or translational products encoded by said nucleic acid segments.
A method of stimulating bone progenitor cells located within a bone progenitor tissue site of an animal, comprising contacting said tissue site with a composition comprising two or more osteotropic genes and a structural bone-compatible matrix so as to promote expression of said genes by said cells.
A method of delivering two or more nucleic acid segments to a fibroblast cell located within a repair tissue site of an animal, comprising contacting said tissue site with a composition comprising two or more nucleic acid segments and a structural bone-compatible matrix to effect uptake of the nucleic acid segments into the fibroblast cell and to promote expression of transcriptional or translational products by said fibroblast cell.
A method of delivering two or more selected nucleic acid segments to a fibroblast cell located within a repair tissue site of an animal, comprising the steps of:
- (a) preparing a matrix-nucleic acid composition comprising two or more nucleic acid segments and a structural bone-compatible matrix;
- and
- (b) contacting said repair tissue site with the structural matrix-nucleic acid composition to effect uptake of the nucleic acid segments by the fibroblast cell, wherein said fibroblast cell expresses transcriptional or translational products encoded by said nucleic acid segments.
A composition comprising two or more nucleic acid segments in association with a structural bone-compatible matrix.
A composition comprising two or more osteotropic genes in association with a structural bone-compatible matrix, said composition being capable of stimulating bone growth when administered to a bone progenitor tissue site of an animal.
An osteotropic device, comprising two or more osteotropic genes capable of expression by bone progenitor cells, the genes associated with an amount of a structural bone-compatible matrix effective to absorb or adsorb said genes, wherein said device is capable of stimulating bone formation when implanted within a bone progenitor tissue site of an animal.
Dependencies are inferred from the claim text.