Recombinant human milk proteins in infant formulas
OwnerCharles W. Slatteryinventor
Life of this patent
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Abstract
A human infant formula sufficient to meet the nutritional requirements of a human infant, comprising proteins having substantially the same amino acid sequence and biological properties as human alpha-lactalbumin and human beta-casein. The proteins may be produced from microorganisms, particularly E. coli. A recombinant DNA segment comprising a human milk protein encoding gene; a promoter sequence directing the transcription of the gene, where the promoter sequence is different from the promoter sequence for the gene in the human organism; and a terminator site for the human milk protein encoding gene. A microorganism containing a recombinant DNA segment comprising a human milk protein encoding gene; a promoter sequence directing the transcription of the gene; and a terminator site for the gene.
Claims11
A method for producing a human infant formula, comprising combining recombinant human .alpha.-lactalbumin, recombinant human .beta.-casein, or both, with lipids from non-human sources, vitamins, minerals, and other nutrients which in combination are sufficient to meet the nutritional requirements of a human infant.
In a method for meeting the nutritional needs of a human infant, in which the infant is fed milk protein, vitamins, minerals, and lipids, the improvement comprising feeding the infant a recombinant human milk protein.
A method for producing a human infant formula, said formula comprising human .alpha.-lactaitumin or recombinant human .beta.-casein, lipids derived from a non-human source, vitamins and minerals sufficient to meet the nutritional requirements of a human infant, comprising:
- inserting at least one promoted, heterologous, expressible recombinant DNA segment encoding said human .alpha.-lactalbumin or human .beta.-casein into a vector to produce a transforming vector;
- transforming a microorganism with said transforming vector;
- obtaining expression of said human .alpha.-lactalbumin or human .beta.-casein by said transformed microorganism;
- harvesting and purifying said human .alpha.-lactalbumin or human .beta.-casein expressed by said microorganism;
- and
- formulating said human infant formula with said human .alpha.-lactalbumin or human .beta.-casein.
A human infant formiula comprising protein, lipids from non-human sources, vitamins and minerals, wherein said protein comprises a recombinant human milk protein produced by a non-human organmism.
Dependencies are inferred from the claim text.