DNA molecules encoding plant protoporphyrinogen oxidase and inhibitor-resistant mutants thereof
AssigneeNovartis Finance Corporation
InventorsSandra L. Volrath, Marie A. Johnson, Eric R. Ward, Peter B. Heifetz
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Abstract
The present invention provides novel DNA sequences coding for plant protoporphyrinogen oxidase (protox) enzymes from soybean, wheat, cotton, sugar beet, rape, rice and sorghum. In addition, the present invention teaches modified forms of protox enzymes that are herbicide tolerant. Plants expressing herbicide tolerant protox enzymes taught herein are also provided. These plants may be engineered for resistance to protox inhibitors via mutation of the native protox gene to a resistant form or they may be transformed with a gene encoding an inhibitor-resistant from of a plant protox enzyme.
Claims144
A modified plant DNA molecule encoding a modified enzyme having protoporphyrinogen oxidase activity, wherein said modified enzyme has at least one amino acid modification compared to a naturally occurring protox enzyme, wherein said at least one amino acid modification confers resistance to an inhibitor of the naturally occurring protox enzyme, and wherein said at least one amino acid modification comprises an amino acid substitution occurring at a position corresponding to position 240, 245, 246, 388, 390, 451, 455, 500, or 536 of the comparative alignment shown in Table 1.
Dependencies are inferred from the claim text.