Strains of Escherichia coli, methods of preparing the same and use thereof in fermentation processes for l-threonine production
AssigneeArcher Daniels Midland Company
InventorsMing-Der Wang, Jill S. Bradshaw, Stacia L. Swisher, Hungming James Liaw, Paul D. Hanke, Thomas P. Binder
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Abstract
The present invention relates to novel strains of Escherichia coli and fermentation processes involving these microorganisms. More specifically, the present invention relates to genetically-modified Escherichia coli strains and the use thereof for the production of the amino acids, particularly members of the aspartate family of amino acids such as threonine. The present invention also relates to methods of preparing E. coli strains for use in the fermentative production of amino acids.
Claims20
A strain of the microorganism E. coli having the following characteristics:
- (i) its chromosome contains at least one threonine (thr) operon operably linked with at least one non-native promoter which replaces the native promoter via insertion into said chromosome;
- (ii) it does not require any recombinant plasmids containing one or more genes that encode one or more of the threonine biosynthesis enzymes in order to produce threonine;
- and
- (iii) it produces L-threonine.
Dependencies are inferred from the claim text.