Catalases
AssigneeDiversa Corporation
InventorsDan E. Robertson, Indrajit Sanyal, Robert S. Adhikary
Life of this patent
Estimating the term…
Abstract
Catalase enzymes derived from bacteria from the genera Alcaligenes (Deleya) and Microscilla are disclosed. The enzymes are produced from native or recombinant host cells and can be utilized to destroy or detect hydrogen peroxide, e.g., in production of glyoxylic acid and in glucose sensors, and in processes where hydrogen peroxide is used as a bleaching or antibacterial agent, e.g. in contact lens cleaning, in bleaching steps in pulp and paper preparation and in the pasteurization of dairy products.
Claims12
An isolated polynucleotide selected from the group consisting of:
- (a) SEQ ID NO:5;
- (b) SEQ ID NO:7;
- (c) SEQ ID NO:5, wherein T can also be U;
- (d) SEQ ID NO:7, wherein T can also be U;
- (e) nucleic acid sequences fully complementary to (a), (b) or (c);
- and
- (f) fragments of (a), (b), (c), or (d) that are at least 15 bases in length and that will hybridize to a nucleic acid sequence encoding the polypeptide set forth in either SEQ ID NO:6 or SEQ ID NO:8 under conditions that include 0.9 M NaCl 5.0 mM NaH.sub.2 PO.sub.4, 5.0 mM Na.sub.2 EDTA, 0.5% SDS and 10.times.Denhardt's at about 45.degree. C.
An isolated polynucleotide selected from the group consisting of:
- (a) a polynucleotide encoding an enzyme comprising an amino acid sequence selected from the group of amino acid sequences set forth in SEQ ID NOS:6 and 8;
- and
- (b) a polynucleotide which is complementary to a polynucleotide of (a).
An isolated polynucleotide as set forth in SEQ ID NO:5.
An isolated polynucleotide as set forth in SEQ ID NO:7.
Dependencies are inferred from the claim text.