Production of tryptophan by microorganisms
AssigneeArcher Daniels Midland Company
InventorsMark S. Dunkak, Richard D. Dancey, Thomas W. McMullin, Paul D. Hanke
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Abstract
An improved recombinant DNA plasmid composed of a DNA vector and other DNA fragments containing the tryptophan operon from E. coli, the aroG gene from E. coli and the serA gene from E. coli is shown. The plasmid is transformed into a microorganism belonging to Escherichia coli, and the microorganisms are cultured in a medium and the L-tryptophan accumulated in the culture is recovered.
Claims11
A bacterium which comprises a host of the species Escherichia coli and a plasmid with the pBR322 origin of replication carrying genes from Escherichia coli for trpEDCBA, aroG, serA and lacl.
A bacterium containing a plasmid selected from the group consisting of p5LRPS2, p5LRPS3, p6LRPS2, p6LRPS3, p5P11A1, p5R21A1 or any combination of said plasmids with tandem lacUV5 promoters or tac promoters controlling a gene selected from the group consisting of trpEDCBA and aroG.
A method of producing tryptophan comprising the steps of culturing a bacterium JB102(p5LRPS2) in medium under high back pressure for a period of time sufficient for said bacterium to produce tryptophan, and removing said tryptophan from the culture medium.
Dependencies are inferred from the claim text.