Ribonuclease resistant RNA preparation and utilization
AssigneesAmbion, Inc., Cenetron Diagnostics, LLC
InventorsBrittan L. Pasloske, Dwight DuBois, David Brown, Matthew Winkler
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Abstract
The present invention relates to nuclease resistant nucleic acids in general and ribonuclease resistant RNAs in particular. Methods of making and using such nucleic acids are disclosed.
Claims64
A method of assaying for the presence of a tested nucleic acid sequence in a sample comprising:
- obtaining a sample;
- obtaining a nucleic acid standard comprising a nuclease resistant nucleic acid segment comprising a segment encoding a sequence that serves as a standard in detection and/or quantification of the tested nucleic acid;
- assaying the sample for the presence of a tested nucleic acid sequence;
- and
- employing the nucleic acid segment comprising the segment encoding a sequence that serves as a standard in detection and/or quantification of the tested nucleic acid as a standard in the assay.
A nucleic acid standard comprising a ribonuclease resistant RNA segment comprising a segment encoding a sequence that can serve as a standard in detection and/or quantification of a tested nucleic acid, wherein the ribonuclease resistant RNA segment is:
- (1) a chemically modified ribonuclease resistant RNA, (2) coated with a ribonuclease resistant coating, or (3) caged in a ribonuclease resistant structure.
A method of assaying for the presence of a tested RNA sequence in a sample comprising:
- obtaining an RNA sample;
- obtaining an RNA standard comprising a nuclease resistant nucleic acid segment comprising a segment encoding a sequence that serves as a standard in detection and/or quantification of the tested nucleic acid, said segment encapsidated in a ribonuclease resistant structure which is comprised of bacteriophage, animal virus or plant virus coat protein;
- assaying the sample for the presence of a tested RNA sequence;
- and
- employing the RNA segment comprising the segment encoding a sequence that serves as a standard in detection and/or quantification of the tested nucleic acid as a standard in the assay.
Dependencies are inferred from the claim text.