Mutated .beta.-lactam acylase genes
AssigneeGist-Brocades N.V.
InventorsWilhelmus Johannes Quax, Onno Misset, Jan Metske Van Der Laan, Herman B. M. Lenting
Life of this patent
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Abstract
New mutant .beta.-lactam acylases are provided exhibiting altered substrate specificities. These .beta.-lactam acylases are obtained by expression of a gene encoding said .beta.-lactam acylase and having an amino acid sequence which differs at least in one amino acid from the wild-type .beta.-lactam acylase.
Claims24
An isolated mutant microorganism Type II .beta.-lactam acylase with altered substrate specificity, wherein said mutant acylase comprises:
- an amino acid substitution at one or more selected sites in said acylase corresponding to an amino acid position selected from the group consisting of residues 60-64 and 177-180 of SY-77 acylase so that altered substrate specificity as compared to a wild-type Type II .beta.-lactam acylase from the same species is obtained.
A method for obtaining a mutant microorganism Type II .beta.-lactam acylase having an altered substrate specificity, said method comprising:
- effecting a mutation in a gene encoding a Type-II .beta.-lactam acylase to produce a mutated gene coding for a mutant .beta.-lactam acylase having an amino acid substitution at one or more selected sites in said acylase corresponding to an amino acid position selected from the group consisting of residues 60-64 and 177-180 of SY-77 acylase, and
- isolating said mutant .beta.-lactam acylase expressed by said mutated gene, wherein said mutant .beta.-lactam acylase exhibits a desired activity change as compared to a wild-type .beta.-lactam acylase from the same species.
Dependencies are inferred from the claim text.