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AssigneeEpoch Pharmaceuticals, Inc.
InventorsRich B. Meyer, Jr., Howard B. Gamper, Igor V. Kutyavin, Alexander A. Gall
Estimating the term…
A method for introducing a site-specific mutation into a target polynucleotide sequence is presented. The method involves the use of an oligonucleotide capable of binding to the target sequence, either by triplex formation (mediated by Hoogsteen, reverse Hoogsteen or equivalent base pairing) or by Watson/Crick base pairing (in the presence of a recombinase enzyme). The oligonucleotide of the invention is modified by the covalent attachment of one or more electrophilic groups. When a modified oligonucleotide is bound to its target sequence, the electrophilic group is able to interact with a nearby nucleotide in the target sequence, causing a modification to the nucleotide that results in a change in nucleotide sequence. Compositions used in the practice of the method are also disclosed.
A method for site-directed mutagenesis of a target in a double-stranded DNA molecule, said double-stranded DNA molecule residing in a cell capable of replicating said double-stranded DNA molecule, said target comprising a sequence of nucleotides, said method comprising the steps of:
The method according to claim 21 wherein one of said two or more electrophilic groups is attached to the 5' terminus of said oligonucleotide.
A method of inducing a mutation in a target, said target comprising a sequence of nucleotides, said method comprising:
Dependencies are inferred from the claim text.