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US 5,935,804 AGranted
Method for detecting eubacteria in biological samples with catalytically inactive murein binding enzymes
OwnerRoger A. Laine, Wai C. J. Loinventors
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Abstract
A method for detecting eubacteria in biological samples with catalytically inactive murein binding enzyme is presented. The biological sample may be chemically treated with alkali to cleave peptide bonds in the sample before incubating the sample with the catalytically inactive murein binding enzyme.
Claims23
A method for detecting the presence or amount of a eubacteria in a biological sample comprising the steps of:
- collecting the biological sample;
- incubating an aliquot of the biological sample with a murein binding polypeptide under conditions suitable for binding of the murein binding polypeptide to the eubacteria;
- detecting the bound murein binding polypeptide thereby detecting the presence or amount of the eubacteria in the biological sample,
- wherein said murein binding polypeptide comprises a catalytically inactive enzyme capable of binding but not cleaving a peptidoglycan comprising NAc-muramic acid in said eubacteria.
1 more element
The method of claim 19, wherein said acetylation reagent comprises acetic anhydride or acetic chloride.
Dependencies are inferred from the claim text.